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Research Article Free access | 10.1172/JCI118786

The expression of ob gene is not acutely regulated by insulin and fasting in human abdominal subcutaneous adipose tissue.

H Vidal, D Auboeuf, P De Vos, B Staels, J P Riou, J Auwerx, and M Laville

INSERM U449, Faculté de Médecine Alexis Carrel, Lyon, France.

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INSERM U449, Faculté de Médecine Alexis Carrel, Lyon, France.

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INSERM U449, Faculté de Médecine Alexis Carrel, Lyon, France.

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INSERM U449, Faculté de Médecine Alexis Carrel, Lyon, France.

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INSERM U449, Faculté de Médecine Alexis Carrel, Lyon, France.

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INSERM U449, Faculté de Médecine Alexis Carrel, Lyon, France.

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INSERM U449, Faculté de Médecine Alexis Carrel, Lyon, France.

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Published July 15, 1996 - More info

Published in Volume 98, Issue 2 on July 15, 1996
J Clin Invest. 1996;98(2):251–255. https://doi.org/10.1172/JCI118786.
© 1996 The American Society for Clinical Investigation
Published July 15, 1996 - Version history
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Abstract

The regulation of ob gene expression in abdominal subcutaneous adipose tissue was investigated using a reverse transcription-competitive PCR method to quantify the mRNA level of leptin. Leptin mRNA level was highly correlated with the body mass index of 26 subjects (12 lean, 7 non-insulin-dependent diabetic, and 7 obese patients). The effect of fasting on ob gene expression was investigated in 10 subjects maintained on a hypocaloric diet (1045 KJ/d) for 5 d. While their metabolic parameters significantly changed (decrease in insulinemia, glycemia, and resting metabolic rate and increase in plasma ketone bodies), the caloric restriction did not modify the leptin mRNA level in the adipose tissue. To verify whether insulin regulates ob gene expression, six lean subjects underwent a 3-h euglycemic hyperinsulinemic (846 +/- 138 pmol/liter) clamp. Leptin and Glut 4 mRNA levels were quantified in adipose tissue biopsies taken before and at the end of the clamp. Insulin infusion produced a significant threefold increase in Glut 4 mRNA while leptin mRNA was not affected. It is concluded that ob gene expression is not acutely regulated by insulin or by metabolic factors related to fasting in human abdominal subcutaneous adipose tissue.

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