Go to JCI Insight
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact
  • Clinical Research and Public Health
  • Current issue
  • Past issues
  • By specialty
    • COVID-19
    • Cardiology
    • Gastroenterology
    • Immunology
    • Metabolism
    • Nephrology
    • Neuroscience
    • Oncology
    • Pulmonology
    • Vascular biology
    • All ...
  • Videos
    • Conversations with Giants in Medicine
    • Video Abstracts
  • Reviews
    • View all reviews ...
    • Complement Biology and Therapeutics (May 2025)
    • Evolving insights into MASLD and MASH pathogenesis and treatment (Apr 2025)
    • Microbiome in Health and Disease (Feb 2025)
    • Substance Use Disorders (Oct 2024)
    • Clonal Hematopoiesis (Oct 2024)
    • Sex Differences in Medicine (Sep 2024)
    • Vascular Malformations (Apr 2024)
    • View all review series ...
  • Viewpoint
  • Collections
    • In-Press Preview
    • Clinical Research and Public Health
    • Research Letters
    • Letters to the Editor
    • Editorials
    • Commentaries
    • Editor's notes
    • Reviews
    • Viewpoints
    • 100th anniversary
    • Top read articles

  • Current issue
  • Past issues
  • Specialties
  • Reviews
  • Review series
  • Conversations with Giants in Medicine
  • Video Abstracts
  • In-Press Preview
  • Clinical Research and Public Health
  • Research Letters
  • Letters to the Editor
  • Editorials
  • Commentaries
  • Editor's notes
  • Reviews
  • Viewpoints
  • 100th anniversary
  • Top read articles
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact
Top
  • View PDF
  • Download citation information
  • Send a comment
  • Terms of use
  • Standard abbreviations
  • Need help? Email the journal
  • Top
  • Abstract
  • Version history
  • Article usage
  • Citations to this article

Advertisement

Research Article Free access | 10.1172/JCI118339

Expression and localization of inducible and endothelial nitric oxide synthase in the rat ovary. Effects of gonadotropin stimulation in vivo.

B J Van Voorhis, K Moore, P J Strijbos, S Nelson, S A Baylis, D Grzybicki, and C P Weiner

Department of Obstetrics and Gynecology, University of Iowa College of Medicine, Iowa City 52242-1080, USA.

Find articles by Van Voorhis, B. in: PubMed | Google Scholar

Department of Obstetrics and Gynecology, University of Iowa College of Medicine, Iowa City 52242-1080, USA.

Find articles by Moore, K. in: PubMed | Google Scholar

Department of Obstetrics and Gynecology, University of Iowa College of Medicine, Iowa City 52242-1080, USA.

Find articles by Strijbos, P. in: PubMed | Google Scholar

Department of Obstetrics and Gynecology, University of Iowa College of Medicine, Iowa City 52242-1080, USA.

Find articles by Nelson, S. in: PubMed | Google Scholar

Department of Obstetrics and Gynecology, University of Iowa College of Medicine, Iowa City 52242-1080, USA.

Find articles by Baylis, S. in: PubMed | Google Scholar

Department of Obstetrics and Gynecology, University of Iowa College of Medicine, Iowa City 52242-1080, USA.

Find articles by Grzybicki, D. in: PubMed | Google Scholar

Department of Obstetrics and Gynecology, University of Iowa College of Medicine, Iowa City 52242-1080, USA.

Find articles by Weiner, C. in: PubMed | Google Scholar

Published December 1, 1995 - More info

Published in Volume 96, Issue 6 on December 1, 1995
J Clin Invest. 1995;96(6):2719–2726. https://doi.org/10.1172/JCI118339.
© 1995 The American Society for Clinical Investigation
Published December 1, 1995 - Version history
View PDF
Abstract

Nitric oxide is reportedly involved in the regulation of several ovarian processes, yet the isoforms of nitric oxide synthase (NOS) expressed in the ovary are unknown. Our purpose was to identify and localize NOS isoenzymes in the rat ovary and to examine++ if mRNA expression of NOS isoenzymes change after gonadotropin stimulation. Using reverse transcriptase-PCR, we demonstrated that inducible (iNOS) and endothelial (eNOS), but not neuronal, NOS mRNAs are expressed in the ovary. In a gonadotropin-stimulated rat model, unstimulated ovaries had the highest levels of iNOS mRNA as quantified by ribonuclease protection assay. After gonadotropin injection, iNOS mRNA declined to undetectable levels in ovaries containing ovulatory follicles before increasing slighty in ovaries containing copora lutea. In situ hybridization studies localized iNOS to granulosa cells of secondary follicles and small antral follicles. Western blots of unstimulated ovaries demonstrated iNOS protein. In contrast to iNOS, eNOS mRNA levels, determined by quantitative PCR, increased after gonadotropin stimulation and peaked in ovaries containing ovulatory follicles before declining in the luteal phase. eNOS protein was localized to blood vessels in the ovary by immunohistochemistry. We conclude that two isoforms of NOS are expressed in the ovary and the mRNA levels for these isozymes are differentially regulated.

Images.

Browse pages

Click on an image below to see the page. View PDF of the complete article

icon of scanned page 2719
page 2719
icon of scanned page 2720
page 2720
icon of scanned page 2721
page 2721
icon of scanned page 2722
page 2722
icon of scanned page 2723
page 2723
icon of scanned page 2724
page 2724
icon of scanned page 2725
page 2725
icon of scanned page 2726
page 2726
Version history
  • Version 1 (December 1, 1995): No description

Article tools

  • View PDF
  • Download citation information
  • Send a comment
  • Terms of use
  • Standard abbreviations
  • Need help? Email the journal

Metrics

  • Article usage
  • Citations to this article

Go to

  • Top
  • Abstract
  • Version history
Advertisement
Advertisement

Copyright © 2025 American Society for Clinical Investigation
ISSN: 0021-9738 (print), 1558-8238 (online)

Sign up for email alerts