Hepatitis B virus (HBV) sequence variation of cytotoxic T lymphocyte epitopes is not common in patients with chronic HBV infection.

B Rehermann; C Pasquinelli; S M Mosier; F V Chisari

doi:10.1172/JCI118191

Department of Molecular and Experimental Medicine, Scripps Research Institute, La Jolla, California 92037, USA.

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Department of Molecular and Experimental Medicine, Scripps Research Institute, La Jolla, California 92037, USA.

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Department of Molecular and Experimental Medicine, Scripps Research Institute, La Jolla, California 92037, USA.

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Department of Molecular and Experimental Medicine, Scripps Research Institute, La Jolla, California 92037, USA.

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Published in Volume 96, Issue 3 on September 1, 1995
J Clin Invest. 1995;96(3):1527–1534. https://doi.org/10.1172/JCI118191.
© 1995 The American Society for Clinical Investigation

Published September 1, 1995 - Version history

It has been suggested that immune selection pressure exerted by the cytotoxic T lymphocyte (CTL) response could be responsible for viral persistence during chronic hepatitis B virus infection. To address this question, in the current study we compared the DNA and amino acid sequences of, and the CTL responses to, multiple HLA-A2-restricted CTL epitopes in the hepatitis B virus in several HLA-A2-positive patients with acute and chronic hepatitis. Our results indicate that the CTL response to these epitopes is barely detectable in the majority of patients with chronic hepatitis. Further, we show that the weak CTL response is not secondary in infection by mutant viruses lacking these epitopes, and we show that the CTL response did not select for escape mutants in any of these patients. We conclude that an ineffective hepatitis B virus specific CTL response is the primary determinant of viral persistence in chronic hepatitis and that immune selection of viral variants is not a common event in the majority of patients.