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Research Article Free access | 10.1172/JCI117788

Monocyte chemotactic protein-1 (MCP-1), -2, and -3 are chemotactic for human T lymphocytes.

D D Taub, P Proost, W J Murphy, M Anver, D L Longo, J van Damme, and J J Oppenheim

Clinical Services Program, Program Resources, Inc., DynCorp, National Cancer Institute-Frederick Cancer Research and Development Center, Maryland 21702.

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Clinical Services Program, Program Resources, Inc., DynCorp, National Cancer Institute-Frederick Cancer Research and Development Center, Maryland 21702.

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Clinical Services Program, Program Resources, Inc., DynCorp, National Cancer Institute-Frederick Cancer Research and Development Center, Maryland 21702.

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Clinical Services Program, Program Resources, Inc., DynCorp, National Cancer Institute-Frederick Cancer Research and Development Center, Maryland 21702.

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Clinical Services Program, Program Resources, Inc., DynCorp, National Cancer Institute-Frederick Cancer Research and Development Center, Maryland 21702.

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Published March 1, 1995 - More info

Published in Volume 95, Issue 3 on March 1, 1995
J Clin Invest. 1995;95(3):1370–1376. https://doi.org/10.1172/JCI117788.
© 1995 The American Society for Clinical Investigation
Published March 1, 1995 - Version history
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Abstract

Monocyte chemotactic protein (MCP)-1, -2, and -3 all have been shown to induce monocyte/macrophage migration in vitro and MCP-1, also known as MCAF, chemoattracts basophils and mast cells. We report here that natural MCP-1 as well as synthetic preparations of MCP-2 and MCP-3 stimulate significant in vitro chemotaxis of human peripheral blood T lymphocytes. This MCP-induced migration was dose-dependent and directional, but not chemokinetic. Phenotypic analysis of the T cell population responsive to MCP-1, MCP-2, and MCP-3 demonstrates that both CD4+ and CD8+ T cells migrated in response to these chemokines. Similar results were observed using human CD4+ and CD8+ T cell clones. Neutralizing antisera to MCAF or MCP-2 abrogated T cell migration in response to MCP-1 and MCP-2, respectively, but not to RANTES. Subcutaneous administration of purified MCP-1 into the hind flanks of SCID mice engrafted with human peripheral blood lymphocytes (PBL) induced significant human CD3+ T cell infiltration into the site of injection at 4 h. These results demonstrate that MCP-1, MCP-2, and MCP-3 are inflammatory mediators that specifically stimulate the directional migration of T cells as well as monocytes and may play an important role in immune cell recruitment into sites of antigenic challenge.

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