Go to JCI Insight
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact
  • Clinical Research and Public Health
  • Current issue
  • Past issues
  • By specialty
    • COVID-19
    • Cardiology
    • Gastroenterology
    • Immunology
    • Metabolism
    • Nephrology
    • Neuroscience
    • Oncology
    • Pulmonology
    • Vascular biology
    • All ...
  • Videos
    • Conversations with Giants in Medicine
    • Video Abstracts
  • Reviews
    • View all reviews ...
    • Complement Biology and Therapeutics (May 2025)
    • Evolving insights into MASLD and MASH pathogenesis and treatment (Apr 2025)
    • Microbiome in Health and Disease (Feb 2025)
    • Substance Use Disorders (Oct 2024)
    • Clonal Hematopoiesis (Oct 2024)
    • Sex Differences in Medicine (Sep 2024)
    • Vascular Malformations (Apr 2024)
    • View all review series ...
  • Viewpoint
  • Collections
    • In-Press Preview
    • Clinical Research and Public Health
    • Research Letters
    • Letters to the Editor
    • Editorials
    • Commentaries
    • Editor's notes
    • Reviews
    • Viewpoints
    • 100th anniversary
    • Top read articles

  • Current issue
  • Past issues
  • Specialties
  • Reviews
  • Review series
  • Conversations with Giants in Medicine
  • Video Abstracts
  • In-Press Preview
  • Clinical Research and Public Health
  • Research Letters
  • Letters to the Editor
  • Editorials
  • Commentaries
  • Editor's notes
  • Reviews
  • Viewpoints
  • 100th anniversary
  • Top read articles
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact
Top
  • View PDF
  • Download citation information
  • Send a comment
  • Terms of use
  • Standard abbreviations
  • Need help? Email the journal
  • Top
  • Abstract
  • Version history
  • Article usage
  • Citations to this article

Advertisement

Research Article Free access | 10.1172/JCI117430

C5a-induced expression of P-selectin in endothelial cells.

K E Foreman, A A Vaporciyan, B K Bonish, M L Jones, K J Johnson, M M Glovsky, S M Eddy, and P A Ward

Parke-Davis Research Division of Warner-Lambert Company, Ann Arbor, Michigan 48105.

Find articles by Foreman, K. in: PubMed | Google Scholar

Parke-Davis Research Division of Warner-Lambert Company, Ann Arbor, Michigan 48105.

Find articles by Vaporciyan, A. in: PubMed | Google Scholar

Parke-Davis Research Division of Warner-Lambert Company, Ann Arbor, Michigan 48105.

Find articles by Bonish, B. in: PubMed | Google Scholar

Parke-Davis Research Division of Warner-Lambert Company, Ann Arbor, Michigan 48105.

Find articles by Jones, M. in: PubMed | Google Scholar

Parke-Davis Research Division of Warner-Lambert Company, Ann Arbor, Michigan 48105.

Find articles by Johnson, K. in: PubMed | Google Scholar

Parke-Davis Research Division of Warner-Lambert Company, Ann Arbor, Michigan 48105.

Find articles by Glovsky, M. in: PubMed | Google Scholar

Parke-Davis Research Division of Warner-Lambert Company, Ann Arbor, Michigan 48105.

Find articles by Eddy, S. in: PubMed | Google Scholar

Parke-Davis Research Division of Warner-Lambert Company, Ann Arbor, Michigan 48105.

Find articles by Ward, P. in: PubMed | Google Scholar

Published September 1, 1994 - More info

Published in Volume 94, Issue 3 on September 1, 1994
J Clin Invest. 1994;94(3):1147–1155. https://doi.org/10.1172/JCI117430.
© 1994 The American Society for Clinical Investigation
Published September 1, 1994 - Version history
View PDF
Abstract

Human umbilical vein endothelial cells have recently been shown to respond to C5a with increases in intracellular Ca2+, production of D-myo-inositol 1,4,5-triphosphate and superoxide anion generation. In the current studies, C5a had been found to cause in a time- and dose-dependent manner rapid expression of endothelial P-selectin, secretion of von Willebrand factor, and adhesiveness for human neutrophils. The effects of C5a in P-selectin expression and adhesiveness of neutrophils were similar to the effects of histamine and thrombin on endothelial cells. The adhesiveness of C5a-stimulated endothelium for neutrophils was blocked by anti-P-selectin, but not by antibodies to intercellular adhesion molecule 1, E-selectin, or CD18. A cell-based ELISA technique has confirmed upregulation of P-selectin in endothelial cells exposed to C5a. Binding of C5a to endothelial cells has been demonstrated, with molecules bound being approximately 10% of those binding to neutrophils. By a reverse transcriptase-PCR technique, endothelial cells have been shown to contain mRNA for the C5a receptor. These data suggest that C5a may be an important inflammatory mediator for the early adhesive interactions between neutrophils and endothelial cells in the acute inflammatory response.

Images.

Browse pages

Click on an image below to see the page. View PDF of the complete article

icon of scanned page 1147
page 1147
icon of scanned page 1148
page 1148
icon of scanned page 1149
page 1149
icon of scanned page 1150
page 1150
icon of scanned page 1151
page 1151
icon of scanned page 1152
page 1152
icon of scanned page 1153
page 1153
icon of scanned page 1154
page 1154
icon of scanned page 1155
page 1155
Version history
  • Version 1 (September 1, 1994): No description

Article tools

  • View PDF
  • Download citation information
  • Send a comment
  • Terms of use
  • Standard abbreviations
  • Need help? Email the journal

Metrics

  • Article usage
  • Citations to this article

Go to

  • Top
  • Abstract
  • Version history
Advertisement
Advertisement

Copyright © 2025 American Society for Clinical Investigation
ISSN: 0021-9738 (print), 1558-8238 (online)

Sign up for email alerts