Autoimmune myocarditis induced in mice by cardiac C-protein. Cloning of complementary DNA encoding murine cardiac C-protein and partial characterization of the antigenic peptides.

H Kasahara; M Itoh; T Sugiyama; N Kido; H Hayashi; H Saito; S Tsukita; N Kato

doi:10.1172/JCI117416

First Department of Internal Medicine, Nagoya University School of Medicine, Japan.

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First Department of Internal Medicine, Nagoya University School of Medicine, Japan.

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First Department of Internal Medicine, Nagoya University School of Medicine, Japan.

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First Department of Internal Medicine, Nagoya University School of Medicine, Japan.

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First Department of Internal Medicine, Nagoya University School of Medicine, Japan.

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First Department of Internal Medicine, Nagoya University School of Medicine, Japan.

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First Department of Internal Medicine, Nagoya University School of Medicine, Japan.

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First Department of Internal Medicine, Nagoya University School of Medicine, Japan.

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Published in Volume 94, Issue 3 on September 1, 1994
J Clin Invest. 1994;94(3):1026–1036. https://doi.org/10.1172/JCI117416.
© 1994 The American Society for Clinical Investigation

Published September 1, 1994 - Version history

Autoimmune myocarditis is considered to play a major role in the pathogenesis of dilated cardiomyopathy. A new autoimmune myocarditis model was attained by repeated immunization using murine cardiac C-protein with the immunological adjuvant, Klebsiella pneumoniae O3 lipopolysaccharide. For further analysis of a pathological epitope, the cDNA encoding C-protein was isolated; a fusion protein encoded by part of this cDNA induced myocarditis in SMA mice as well as in three other strains: DBA/1J (H-2q), O20/A (H-2pz1), and SJL (H-2s). The nucleotide sequence and its deduced amino acid analysis revealed that this protein had immunoglobulin-like and fibronectin-like repeats. This study provides a new animal model of autoimmune myocarditis which may shed light on the pathogenesis of dilated cardiomyopathy.

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