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Research Article Free access | 10.1172/JCI117219
Department of Pharmacology, College of Physicians and Surgeons of Columbia University, New York 10032.
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Department of Pharmacology, College of Physicians and Surgeons of Columbia University, New York 10032.
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Department of Pharmacology, College of Physicians and Surgeons of Columbia University, New York 10032.
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Department of Pharmacology, College of Physicians and Surgeons of Columbia University, New York 10032.
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Department of Pharmacology, College of Physicians and Surgeons of Columbia University, New York 10032.
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Department of Pharmacology, College of Physicians and Surgeons of Columbia University, New York 10032.
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Department of Pharmacology, College of Physicians and Surgeons of Columbia University, New York 10032.
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Department of Pharmacology, College of Physicians and Surgeons of Columbia University, New York 10032.
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Department of Pharmacology, College of Physicians and Surgeons of Columbia University, New York 10032.
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Published May 1, 1994 - More info
We have shown that the heart expresses two distinct forms of adenylylcyclase mRNA, types V and VI. In this study we have characterized the expression of these two mRNA species in heart failure generated by overdrive pacing at a rate of 240 beats/min. After 4 wk, left ventricular end-diastolic pressure and heart rate increased significantly with the appearance of signs of heart failure, i.e., edema, ascites, and exercise intolerance. Basal as well as forskolin-stimulated adenylylcyclase activities decreased significantly, which was accompanied by a reduction in the steady state mRNA levels of adenylylcyclase types V and VI. These data suggest that in this model of cardiomyopathy, the downregulation of adenylylcyclase catalytic activity results, at least in part, from a reduction in the steady state levels of types V and VI adenylylcyclase mRNA levels.
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