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Research Article Free access | 10.1172/JCI115625

Molecular cloning of the human Goodpasture antigen demonstrates it to be the alpha 3 chain of type IV collagen.

N Turner, P J Mason, R Brown, M Fox, S Povey, A Rees, and C D Pusey

Department of Medicine, Royal Postgraduate Medical School, London, United Kingdom.

Find articles by Turner, N. in: PubMed | Google Scholar

Department of Medicine, Royal Postgraduate Medical School, London, United Kingdom.

Find articles by Mason, P. in: PubMed | Google Scholar

Department of Medicine, Royal Postgraduate Medical School, London, United Kingdom.

Find articles by Brown, R. in: PubMed | Google Scholar

Department of Medicine, Royal Postgraduate Medical School, London, United Kingdom.

Find articles by Fox, M. in: PubMed | Google Scholar

Department of Medicine, Royal Postgraduate Medical School, London, United Kingdom.

Find articles by Povey, S. in: PubMed | Google Scholar

Department of Medicine, Royal Postgraduate Medical School, London, United Kingdom.

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Department of Medicine, Royal Postgraduate Medical School, London, United Kingdom.

Find articles by Pusey, C. in: PubMed | Google Scholar

Published February 1, 1992 - More info

Published in Volume 89, Issue 2 on February 1, 1992
J Clin Invest. 1992;89(2):592–601. https://doi.org/10.1172/JCI115625.
© 1992 The American Society for Clinical Investigation
Published February 1, 1992 - Version history
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Abstract

To characterize the autoantigen of Goodpasture's (anti-glomerular basement membrane) disease, a molecule of 26-kD reactive with autoantibodies from patients' sera was purified from collagenase digests of sheep glomerular basement membrane. Short internal amino acid sequences were obtained after tryptic or cyanogen bromide cleavage, and used to deduce redundant oligonucleotides for use in the polymerase chain reaction on cDNA derived from sheep renal cortex. Molecules of 175 bp were amplified and found to come from two cDNA sequences. One was identical to that of a type IV collagen chain (alpha 5) cloned from human placenta and shown to be expressed in human kidney. The other was from a type IV collagen chain with close similarities to alpha 1 and alpha 5 chains, and was used to obtain human cDNA sequences by cDNA library screening and by further polymerase chain reaction amplifications. The correspondence of the derived amino acid sequence of the new chain with published protein and cDNA sequences shows it to be the alpha 3 chain of type IV collagen. Its gene, COL4A3, maps to 2q36-2q37. The primary sequence and other characteristics of this chain confirm that it carries the Goodpasture antigen.

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