Go to JCI Insight
Jci spelled out white on transparent.20160208
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Alerts
  • Advertising/recruitment
  • Subscribe
  • Contact
  • Current Issue
  • Past Issues
  • By specialty
    • Cardiology
    • Gastroenterology
    • Immunology
    • Metabolism
    • Nephrology
    • Neuroscience
    • Oncology
    • Pulmonology
    • Vascular biology
    • All...
  • Videos
    • Conversations with Giants in Medicine
    • Author's Takes
  • Reviews
    • View all reviews...
    • Biology of familial cancer predisposition syndromes (Feb 2019)
    • Mitochondrial dysfunction in disease (Aug 2018)
    • Lipid mediators of disease (Jul 2018)
    • Cellular senescence in human disease (Apr 2018)
    • Fibrosis (Jan 2018)
    • Glia and Neurodegeneration (Sep 2017)
    • Transplantation (Jun 2017)
    • View all review series...
  • Collections
    • Recently published
    • In-Press Preview
    • Commentaries
    • Concise Communication
    • Editorials
    • Viewpoint
    • Scientific Show Stoppers
    • Top read articles
  • Clinical Medicine
  • JCI This Month
    • Current issue
    • Past issues

Jci only white

  • About
  • Editors
  • Consulting Editors
  • For authors
  • Current issue
  • Past issues
  • By specialty
  • Subscribe
  • Alerts
  • Advertise
  • Contact
  • Conversations with Giants in Medicine
  • Author's Takes
  • Recently published
  • Brief Reports
  • Technical Advances
  • Commentaries
  • Editorials
  • Hindsight
  • Review series
  • Reviews
  • The Attending Physician
  • First Author Perspectives
  • Scientific Show Stoppers
  • Top read articles
  • Concise Communication
Top
  • View PDF Adobe pdf file icon
  • Download citation information
  • Send a letter
  • License information
  • Standard abbreviations
  • Article usage
  • Citations to this article
  • Share this article
  • Need Help? E-mail the JCI
  • Top
  • Abstract
  • Version history
Advertisement

Research Article Free access | 10.1172/JCI115532

Monocyte transmigration induced by modification of low density lipoprotein in cocultures of human aortic wall cells is due to induction of monocyte chemotactic protein 1 synthesis and is abolished by high density lipoprotein.

M Navab, S S Imes, S Y Hama, G P Hough, L A Ross, R W Bork, A J Valente, J A Berliner, D C Drinkwater, and H Laks

Department of Medicine, University of California Los Angeles 90024-167917.

Find articles by Navab, M. in: JCI | PubMed | Google Scholar

Department of Medicine, University of California Los Angeles 90024-167917.

Find articles by Imes, S. in: JCI | PubMed | Google Scholar

Department of Medicine, University of California Los Angeles 90024-167917.

Find articles by Hama, S. in: JCI | PubMed | Google Scholar

Department of Medicine, University of California Los Angeles 90024-167917.

Find articles by Hough, G. in: JCI | PubMed | Google Scholar

Department of Medicine, University of California Los Angeles 90024-167917.

Find articles by Ross, L. in: JCI | PubMed | Google Scholar

Department of Medicine, University of California Los Angeles 90024-167917.

Find articles by Bork, R. in: JCI | PubMed | Google Scholar

Department of Medicine, University of California Los Angeles 90024-167917.

Find articles by Valente, A. in: JCI | PubMed | Google Scholar

Department of Medicine, University of California Los Angeles 90024-167917.

Find articles by Berliner, J. in: JCI | PubMed | Google Scholar

Department of Medicine, University of California Los Angeles 90024-167917.

Find articles by Drinkwater, D. in: JCI | PubMed | Google Scholar

Department of Medicine, University of California Los Angeles 90024-167917.

Find articles by Laks, H. in: JCI | PubMed | Google Scholar

First published December 1, 1991 - More info

Published in Volume 88, Issue 6 on December 1, 1991
J Clin Invest. 1991;88(6):2039–2046. https://doi.org/10.1172/JCI115532.
Copyright © 1991, The American Society for Clinical Investigation.

First published December 1, 1991 - Version history
Abstract

Incubation of cocultures of human aortic endothelial (HAEC) and smooth muscle cells (HASMC) with LDL in the presence of 5-10% human serum resulted in a 7.2-fold induction of mRNA for monocyte chemotactic protein 1 (MCP-1), a 2.5-fold increase in the levels of MCP-1 protein in the coculture supernatants, and a 7.1-fold increase in the transmigration of monocytes into the subendothelial space of the cocultures. Monocyte migration was inhibited by 91% by antibody to MCP-1. Media collected from the cocultures that had been incubated with LDL induced target endothelial cells (EC) to bind monocyte but not neutrophil-like cells. Media collected from cocultures that had been incubated with LDL-induced monocyte migration into the subendothelial space of other cocultures that had not been exposed to LDL. In contrast, media from separate cultures of EC or smooth muscle cells (SMC) containing equal number of EC or SMC compared to coculture and incubated with the same LDL did not induce monocyte migration when incubated with the target cocultures. High density lipoprotein HDL, when presented to cocultures together with LDL, reduced the increased monocyte transmigration by 91%. Virtually all of the HDL-mediated inhibition was accounted for by the HDL2 subfraction. HDL3 was essentially without effect. Apolipoprotein AI was also ineffective in preventing monocyte transmigration while phosphatidylcholine liposomes were as effective as HDL2 suggesting that lipid components of HDL2 may have been responsible for its action. Preincubating LDL with beta-carotene or with alpha-tocopherol did not reduce monocyte migration. However, pretreatment of LDL with probucol or pretreatment of the cocultures with probucol, beta-carotene, or alpha-tocopherol before the addition of LDL prevented the LDL-induced monocyte transmigration. Addition of HDL or probucol to LDL after the exposure to cocultures did not prevent the modified LDL from inducing monocyte transmigration in fresh cocultures. We conclude that cocultures of human aortic cells can modify LDL even in the presence of serum, resulting in the induction of MCP-1, and that HDL and antioxidants prevent the LDL induced monocyte transmigration.

Images
Browse pages

Click on an image below to see the page. View PDF of the complete article

icon of scanned page 2039
page 2039
icon of scanned page 2040
page 2040
icon of scanned page 2041
page 2041
icon of scanned page 2042
page 2042
icon of scanned page 2043
page 2043
icon of scanned page 2044
page 2044
icon of scanned page 2045
page 2045
icon of scanned page 2046
page 2046
Version history
  • Version 1 (December 1, 1991): No description

Article tools

  • View PDF Adobe pdf file icon
  • Download citation information
  • Send a letter
  • License information
  • Standard abbreviations
  • Article usage
  • Citations to this article
  • Share this article
  • Need Help? E-mail the JCI

Go to:

  • Top
  • Abstract
  • Version history
Advertisement
Advertisement
Follow JCI: Facebook logo white Twitter logo v2 Rss icon
Copyright © 2019 American Society for Clinical Investigation
ISSN: 0021-9738 (print), 1558-8238 (online)

Sign up for email alerts