Advertisement

Research Article Free access | 10.1172/JCI115523

Surfactant chemical composition and biophysical activity in acute respiratory distress syndrome.

T J Gregory, W J Longmore, M A Moxley, J A Whitsett, C R Reed, A A Fowler 3rd, L D Hudson, R J Maunder, C Crim, and T M Hyers

Medical Department, Ross Laboratories Division of Abbott Laboratories, Columbus, Ohio 43215.

Find articles by Gregory, T. in: JCI | PubMed | Google Scholar

Medical Department, Ross Laboratories Division of Abbott Laboratories, Columbus, Ohio 43215.

Find articles by Longmore, W. in: JCI | PubMed | Google Scholar

Medical Department, Ross Laboratories Division of Abbott Laboratories, Columbus, Ohio 43215.

Find articles by Moxley, M. in: JCI | PubMed | Google Scholar

Medical Department, Ross Laboratories Division of Abbott Laboratories, Columbus, Ohio 43215.

Find articles by Whitsett, J. in: JCI | PubMed | Google Scholar

Medical Department, Ross Laboratories Division of Abbott Laboratories, Columbus, Ohio 43215.

Find articles by Reed, C. in: JCI | PubMed | Google Scholar

Medical Department, Ross Laboratories Division of Abbott Laboratories, Columbus, Ohio 43215.

Find articles by Fowler, A. in: JCI | PubMed | Google Scholar

Medical Department, Ross Laboratories Division of Abbott Laboratories, Columbus, Ohio 43215.

Find articles by Hudson, L. in: JCI | PubMed | Google Scholar

Medical Department, Ross Laboratories Division of Abbott Laboratories, Columbus, Ohio 43215.

Find articles by Maunder, R. in: JCI | PubMed | Google Scholar

Medical Department, Ross Laboratories Division of Abbott Laboratories, Columbus, Ohio 43215.

Find articles by Crim, C. in: JCI | PubMed | Google Scholar

Medical Department, Ross Laboratories Division of Abbott Laboratories, Columbus, Ohio 43215.

Find articles by Hyers, T. in: JCI | PubMed | Google Scholar

Published December 1, 1991 - More info

Published in Volume 88, Issue 6 on December 1, 1991
J Clin Invest. 1991;88(6):1976–1981. https://doi.org/10.1172/JCI115523.
© 1991 The American Society for Clinical Investigation
Published December 1, 1991 - Version history
View PDF
Abstract

Acute Respiratory Distress Syndrome (ARDS) is characterized by lung injury and damage to the alveolar type II cells. This study sought to determine if endogenous surfactant is altered in ARDS. Bronchoalveolar lavage was performed in patients at-risk to develop ARDS (AR, n = 20), with ARDS (A, n = 66) and in normal subjects (N, n = 29). The crude surfactant pellet was analyzed for total phospholipids (PL), individual phospholipids, SP-A, SP-B, and minimum surface tension (STmin). PL was decreased in both AR and A (3.48 +/- 0.61 and 2.47 +/- 0.40 mumol/ml, respectively) compared to N (7.99 +/- 0.60 mumol/ml). Phosphatidylcholine was decreased in A (62.64 +/- 2.20% PL) compared to N (76.27 +/- 2.05% PL). Phosphatidylglycerol was 11.58 +/- 1.21% PL in N and was decreased to 6.48 +/- 1.43% PL in A. SP-A was 123.64 +/- 20.66 micrograms/ml in N and was decreased to 49.28 +/- 21.68 micrograms/ml in AR and to 29.88 +/- 8.49 micrograms/ml in A. SP-B was 1.28 +/- 0.33 micrograms/ml in N and was decreased to 0.57 +/- 0.24 micrograms/ml in A. STmin was increased in AR (15.1 +/- 2.53 dyn/cm) and A (29.04 +/- 2.05 dyn/cm) compared to N (7.44 +/- 1.61 dyn/cm). These data demonstrate that the chemical composition and functional activity of surfactant is altered in ARDS. Several of these alterations also occur in AR, suggesting that these abnormalities occur early in the disease process.

Browse pages

Click on an image below to see the page. View PDF of the complete article

Version history
  • Version 1 (December 1, 1991): No description
Advertisement
Advertisement