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Research Article Free access | 10.1172/JCI115281

Interleukin 4 suppresses the spontaneous growth of chronic myelomonocytic leukemia cells.

K Akashi, T Shibuya, M Harada, Y Takamatsu, N Uike, T Eto, and Y Niho

First Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.

Find articles by Akashi, K. in: PubMed | Google Scholar

First Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.

Find articles by Shibuya, T. in: PubMed | Google Scholar

First Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.

Find articles by Harada, M. in: PubMed | Google Scholar

First Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.

Find articles by Takamatsu, Y. in: PubMed | Google Scholar

First Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.

Find articles by Uike, N. in: PubMed | Google Scholar

First Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.

Find articles by Eto, T. in: PubMed | Google Scholar

First Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.

Find articles by Niho, Y. in: PubMed | Google Scholar

Published July 1, 1991 - More info

Published in Volume 88, Issue 1 on July 1, 1991
J Clin Invest. 1991;88(1):223–230. https://doi.org/10.1172/JCI115281.
© 1991 The American Society for Clinical Investigation
Published July 1, 1991 - Version history
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Abstract

We studied the effects of IL-4 on the spontaneous proliferation of chronic myelomonocytic leukemia (CMMoL) cells in vitro. IL-4 (100 U/ml) suppressed the spontaneous DNA synthesis by approximately 50% in 5 of 8 cases examined. IL-4 (100 U/ml) also inhibited the spontaneous colony formation by CMMoL cells in a methylcellulose culture by 50-97% in all of the 10 cases in which spontaneous colonies were formed. This IL-4-mediated suppression of the growth of CMMoL cells was completely abolished by the addition of anti-IL-4 neutralizing antibodies. The spontaneous CMMoL colonies were substantially suppressed by the addition of either anti-IL-6 or anti-granulocyte/macrophage colony-stimulating factor (GM-CSF) antibodies to the colony assay system: the addition of both anti-IL-6 and anti-GM-CSF antibodies resulted in greater than 80% inhibition of the colony formation by CMMoL cells. On the other hand, none of anti-IL-1-beta, anti-granulocyte-CSF, anti-macrophage-CSF, or anti-tumor necrosis factor-alpha antibodies affected the CMMoL colony formation. In the supernatants from 24-h cultures of CMMoL cells, high levels of IL-6 and GM-CSF were demonstrated in 9 of 9 and 2 of 9 cases examined, respectively. IL-4 (100 U/ml) almost completely inhibited the secretion of IL-6 and GM-CSF by CMMoL cells. These observations suggest that IL-4 suppresses the spontaneous proliferation of CMMoL cells by inhibiting their production of IL-6 and/or GM-CSF, both of which could act in vitro as an autocrine growth factor for CMMoL cells.

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