Usage Information

Insulin-like growth factor I mediates selective anabolic effects of parathyroid hormone in bone cultures.
E Canalis, … , W Burch, T L McCarthy
E Canalis, … , W Burch, T L McCarthy
Published January 1, 1989
Citation Information: J Clin Invest. 1989;83(1):60-65. https://doi.org/10.1172/JCI113885.
View: Text | PDF
Research Article

Insulin-like growth factor I mediates selective anabolic effects of parathyroid hormone in bone cultures.

Abstract

PTH was studied for its effects on bone formation in cultured rat calvariae. 0.01-10 nM PTH stimulated [3H]thymidine incorporation into DNA by up to 4.8-fold. Although continuous treatment with PTH for 24-72 h inhibited [3H]proline incorporation into collagen, transient (24 h) treatment enhanced [3H]proline incorporation into collagen 24-48 h after the hormone was removed. The collagen stimulated by PTH was type I and the effect was observed in the periosteum-free bone and was not blocked by hydroxyurea. Furthermore, treatment with 1-100 nM PTH for 24 h increased insulin-like growth factor (IGF) I concentrations by two to fourfold, and an IGF I antibody prevented the PTH stimulation of collagen synthesis, but not its mitogenic effect. In conclusion, continuous treatment with PTH inhibits calvarial collagen, whereas transient treatment stimulates collagen synthesis, and the stimulatory effect is mediated by local production of IGF I.

Authors

E Canalis, M Centrella, W Burch, T L McCarthy

×

Usage data is cumulative from July 2024 through July 2025.

Usage JCI PMC
Text version 401 9
PDF 115 10
Scanned page 283 1
Citation downloads 130 0
Totals 929 20
Total Views 949
(Click and drag on plot area to zoom in. Click legend items above to toggle)

Usage information is collected from two different sources: this site (JCI) and Pubmed Central (PMC). JCI information (compiled daily) shows human readership based on methods we employ to screen out robotic usage. PMC information (aggregated monthly) is also similarly screened of robotic usage.

Various methods are used to distinguish robotic usage. For example, Google automatically scans articles to add to its search index and identifies itself as robotic; other services might not clearly identify themselves as robotic, or they are new or unknown as robotic. Because this activity can be misinterpreted as human readership, data may be re-processed periodically to reflect an improved understanding of robotic activity. Because of these factors, readers should consider usage information illustrative but subject to change.

Advertisement