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Research Article Free access | 10.1172/JCI112990

Conversion of xanthine dehydrogenase to oxidase in ischemic rat tissues.

T D Engerson, T G McKelvey, D B Rhyne, E B Boggio, S J Snyder, and H P Jones

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Published June 1, 1987 - More info

Published in Volume 79, Issue 6 on June 1, 1987
J Clin Invest. 1987;79(6):1564–1570. https://doi.org/10.1172/JCI112990.
© 1987 The American Society for Clinical Investigation
Published June 1, 1987 - Version history
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Abstract

In response to global ischemia, tissue xanthine dehydrogenase was converted to xanthine oxidase in all tissues with half-times of conversion at 37 degrees C of approximately 3.6, 6, 7, and 14 h for the liver, kidney, heart, and lung, respectively. The time course of enzyme conversion at 4 degrees C was greatly extended with half-conversion times of 6, 5, 5, and 6 d for the respective tissues. Increases in xanthine oxidase activity were accompanied by the appearance of a distinct new protein species with greater electrophoretic mobility. The oxidase from ischemic rat liver was purified 781-fold and found to migrate with a higher mobility on native gels than the purified native dehydrogenase. Sodium dodecyl sulfate profiles revealed the presence of a single major band of 137 kD for the native dehydrogenase, whereas the oxidase had been partially cleaved generating polypeptides of 127, 91, and 57 kD. Polypeptide patterns for the oxidase resemble those seen following limited in vitro proteolysis of the native dehydrogenase supporting a proteolytic mechanism for the conversion of xanthine dehydrogenase to oxidase in ischemic rat liver.

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