Epitopes of apolipoprotein B-100 and B-48 in both liver and intestine. Expression and evidence for local synthesis in recessive abetalipoproteinemia.

R P Dullaart; B Speelberg; H J Schuurman; R W Milne; L M Havekes; Y L Marcel; H J Geuze; M M Hulshof; D W Erkelens

doi:10.1172/JCI112727

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Published in Volume 78, Issue 5 on November 1, 1986
J Clin Invest. 1986;78(5):1397–1404. https://doi.org/10.1172/JCI112727.
© 1986 The American Society for Clinical Investigation

Published November 1, 1986 - Version history

The presence of apolipoprotein (apo) B in liver and intestine from a patient with abetalipoproteinemia was evaluated by immunohistochemistry with a polyclonal and six monoclonal antibodies to different apo B-48 and B-100 epitopes. In normal liver, apo B was present inside and outside hepatocytes. The patients liver exhibited staining in the cytoplasm with the polyclonal and three monoclonal antibodies. By immunoelectron-microscopy, apo B was found to be present in the smooth endoplasmatic reticulum and the Golgi complex. Normal intestinal epithelium was labeled with polyclonal and all monoclonal antibodies, including those specific for apo B-100. The patients epithelium stained with polyclonal and six monoclonals, and apo B was present in the Golgi complex. Thus, normal intestinal mucosa expressed apo B-48 and B-100 epitopes, which indicates apo B-100 synthesis in the gut. The synthesis of the apo B molecule in the patient seems to be retained in both liver and gut, which suggests a posttranslational defect.

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Epitopes of apolipoprotein B-100 and B-48 in both liver and intestine. Expression and evidence for local synthesis in recessive abetalipoproteinemia.

R P Dullaart, B Speelberg, H J Schuurman, R W Milne, L M Havekes, Y L Marcel, H J Geuze, M M Hulshof, and D W Erkelens

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Epitopes of apolipoprotein B-100 and B-48 in both liver and intestine. Expression and evidence for local synthesis in recessive abetalipoproteinemia.

R P Dullaart, B Speelberg, H J Schuurman, R W Milne, L M Havekes, Y L Marcel, H J Geuze, M M Hulshof, and D W Erkelens

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