Selective elimination of anti-DNA antibody-producing cells by antiidiotypic antibody conjugated with neocarzinostatin.

T Sasaki; T Muryoi; O Takai; E Tamate; Y Ono; Y Koide; N Ishida; K Yoshinaga

doi:10.1172/JCI112444

Selective elimination of anti-DNA antibody-producing cells by antiidiotypic antibody conjugated with neocarzinostatin.

T Sasaki, T Muryoi, O Takai, E Tamate, Y Ono, Y Koide, N Ishida, and K Yoshinaga

Published April 1, 1986 - More info

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Abstract

A new strategy was shown for the manipulation of autoantibody production in humans. Antiidiotypic antibody to human anti-DNA autoantibody was conjugated with neocarzinostatin (NCS), a cytotoxic agent, by using N-succimidyl 3-(2-pyridyldithio) propionate as a coupling agent. Human B cell clones, which produce anti-DNA autoantibodies, were killed by in vitro treatment with antiidiotype (Id)-NCS conjugates, while clones expressing an Id with irrelevant specificity were unaffected. These results indicate that treatment with anti-Id-NCS conjugates can act as a potent and specific means of generating immunosuppression of autoantibody production. This approach will have a significant advantage in aborting clones that are not effectively suppressed for the autoantibodies by anti-Id antibodies alone, and will result in a potential therapeutic treatment for systemic lupus erythematosus.

Selective elimination of anti-DNA antibody-producing cells by antiidiotypic antibody conjugated with neocarzinostatin.

T Sasaki, T Muryoi, O Takai, E Tamate, Y Ono, Y Koide, N Ishida, and K Yoshinaga

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Selective elimination of anti-DNA antibody-producing cells by antiidiotypic antibody conjugated with neocarzinostatin.

T Sasaki, T Muryoi, O Takai, E Tamate, Y Ono, Y Koide, N Ishida, and K Yoshinaga

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