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Research Article Free access | 10.1172/JCI111527

Tumor promoters stimulate hyperplasia of microtubule organizing center and inhibit DNA synthesis in cultured cells.

R N Mascardo and P Sherline

Find articles by Mascardo, R. in: PubMed | Google Scholar

Find articles by Sherline, P. in: PubMed | Google Scholar

Published October 1, 1984 - More info

Published in Volume 74, Issue 4 on October 1, 1984
J Clin Invest. 1984;74(4):1186–1192. https://doi.org/10.1172/JCI111527.
© 1984 The American Society for Clinical Investigation
Published October 1, 1984 - Version history
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Abstract

Chemical tumor promoters induce significant morphologic changes in several cultured cell models. In this article we describe a new effect of two potent, chemically different tumor promoters, 12-O-tetradecanoylphorbol-13-acetate (TPA) and dihydroteleocidin B (DHTB) on cultured human HeLa and melanoma cells. Using immunofluorescence microscopy, we observed that TPA and DHTB induced a dramatic increase in the size (greater than or equal to 3X normal diameter) of the centrosome, a microtubule-organizing center, within 24 h of incubation. In HeLa cells the effect was serum- and dose-dependent, was observed in 76-92% of cells within 72 h of incubation, and was associated with an increase in cytoplasm-nucleus ratio and proliferation of microtubules from the centrosome. The tumor promoters inhibited serum-induced DNA synthesis in both cell lines. Electron microscopy revealed the presence of clumps of microcentriole bodies or fragments adjacent to the intact centriole.

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