Advertisement
Research Article Free access | 10.1172/JCI110552
Find articles by Smith, R. in: JCI | PubMed | Google Scholar
Find articles by Koenig, R. in: JCI | PubMed | Google Scholar
Find articles by Binnerts, A. in: JCI | PubMed | Google Scholar
Find articles by Soeldner, J. in: JCI | PubMed | Google Scholar
Find articles by Aoki, T. in: JCI | PubMed | Google Scholar
Published May 1, 1982 - More info
The formation of hemoglobin AIc was studied in intact human erythrocytes in vitro. Satisfactory methods were developed for maintaining erythrocytes under physiologic conditions for greater than 8 d with less than 10% hemolysis. Hemoglobin AIc levels were determined chromatographically on erythrocyte hemolysates after removal of reversible components by incubation for 6 h at 37 degree C. Hemoglobin AIc concentration was found to increase linearly with time during 8 d of incubation. The rate of formation of hemoglobin AIc increased linearly as glucose concentration was increased from 40 to 1,000 mg/dl. Deoxyhemoglobin was glycosylated twice as rapidly as oxyhemoglobin. The rate of hemoglobin AIc formation was further increased by elevated 2,3-diphosphoglycerate levels, an effect that was most marked with deoxyhemoglobin. We conclude that the nonenzymatic glycosylation of hemoglobin is influenced by factors other than glucose, including oxygen tension and 2,3-diphosphoglycerate levels.