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Research Article Free access | 10.1172/JCI110102

Phosphatidylinositol-specific Phospholipase C in Fetal Membranes and Uterine Decidua

Gian Carlo Di Renzo, John M. Johnston, Takeshi Okazaki, Janice R. Okita, Paul C. MacDonald, and John E. Bleasdale

Cecil H. and Ida Green Center for Reproductive Biology Sciences, The University of Texas Southwestern Medical School, Dallas, Texas 75235

Department of Biochemistry, The University of Texas Southwestern Medical School, Dallas, Texas 75235

Department of Obstetrics-Gynecology, The University of Texas Southwestern Medical School, Dallas, Texas 75235

Find articles by Di Renzo, G. in: PubMed | Google Scholar

Cecil H. and Ida Green Center for Reproductive Biology Sciences, The University of Texas Southwestern Medical School, Dallas, Texas 75235

Department of Biochemistry, The University of Texas Southwestern Medical School, Dallas, Texas 75235

Department of Obstetrics-Gynecology, The University of Texas Southwestern Medical School, Dallas, Texas 75235

Find articles by Johnston, J. in: PubMed | Google Scholar

Cecil H. and Ida Green Center for Reproductive Biology Sciences, The University of Texas Southwestern Medical School, Dallas, Texas 75235

Department of Biochemistry, The University of Texas Southwestern Medical School, Dallas, Texas 75235

Department of Obstetrics-Gynecology, The University of Texas Southwestern Medical School, Dallas, Texas 75235

Find articles by Okazaki, T. in: PubMed | Google Scholar

Cecil H. and Ida Green Center for Reproductive Biology Sciences, The University of Texas Southwestern Medical School, Dallas, Texas 75235

Department of Biochemistry, The University of Texas Southwestern Medical School, Dallas, Texas 75235

Department of Obstetrics-Gynecology, The University of Texas Southwestern Medical School, Dallas, Texas 75235

Find articles by Okita, J. in: PubMed | Google Scholar

Cecil H. and Ida Green Center for Reproductive Biology Sciences, The University of Texas Southwestern Medical School, Dallas, Texas 75235

Department of Biochemistry, The University of Texas Southwestern Medical School, Dallas, Texas 75235

Department of Obstetrics-Gynecology, The University of Texas Southwestern Medical School, Dallas, Texas 75235

Find articles by MacDonald, P. in: PubMed | Google Scholar

Cecil H. and Ida Green Center for Reproductive Biology Sciences, The University of Texas Southwestern Medical School, Dallas, Texas 75235

Department of Biochemistry, The University of Texas Southwestern Medical School, Dallas, Texas 75235

Department of Obstetrics-Gynecology, The University of Texas Southwestern Medical School, Dallas, Texas 75235

Find articles by Bleasdale, J. in: PubMed | Google Scholar

Published March 1, 1981 - More info

Published in Volume 67, Issue 3 on March 1, 1981
J Clin Invest. 1981;67(3):847–856. https://doi.org/10.1172/JCI110102.
© 1981 The American Society for Clinical Investigation
Published March 1, 1981 - Version history
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Abstract

An assay procedure was developed in which phosphatidyl[2-3H]inositol was employed as substrate for the measurement of phosphatidylinositol-specific phospholipase C activity. Employing this assay, phosphatidylinositol-specific phospholipase C activity in human fetal membranes and uterine decidua was identified and characterized. The specific activity of this enzyme in amnion (4.4 μmol × mg−1 protein × h−1) was three times that in uterine decidua and more than five times that in chorion laeve. No difference was found between the specific activity of phosphatidylinositol-specific phospholipase C in placental amnion and that in reflected amnion. The products of phosphatidylinositol hydrolysis in short-term incubations were stoichiometric amounts of diacylglycerol and inositol-1,2-cyclic-phosphate plus inositol-1-phosphate. After longer periods of incubation, monoacylglycerol also was detected. Diacylglycerol lipase activity also was demonstrated in these tissues. More than 90% of phosphatidylinositol-specific phospholipase C activity of amnion tissue was recovered in the 105,000-g supernatant fraction, and optimal enzymatic activity in vitro was observed at pH 6.5-7.5 in the presence of Ca2+ (8 mM) and mercaptoethanol (4 mM). Phosphatidylinositol-specific phospholipase C activity was stimulated by fatty acids in low concentrations, but was inhibited by lysophosphatidylcholine and a variety of detergents. No effect of labor on the specific activity of phosphatidylinositol-specific phospholipase C in either fetal membranes or uterine decidua could be detected. The finding of an active phosphatidylinositol-specific phospholipase C activity in human fetal membranes and uterine decidua is complementary to our previous finding of a selective loss of arachidonic acid from phosphatidylinositol of human fetal membranes during labor. The action of phosphatidylinositol-specific phospholipase C, coupled to diacylglycerol lipase action, could provide a mechanism for the release of arachidonic acid for prostaglandin biosynthesis during parturition.

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