Immunologic and Neuropharmacologic Stimulation of Mucous Glycoprotein Release from Human Airways In Vitro

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Abstract

Human bronchial airways obtained after surgical resection were maintained in tissue culture for 24-48 h. Incorporation of [3H]- or [14C]-glucosamine, [14C]threonine, or Na2[35S]O4 to the culture media resulted in biosynthesis of two radiolabeled glycoproteins—one filtering in the exclusion volume of Sepharose 2B, and the other filtering with an approximate molecular weight of 400,000. Both fractions had similar elution patterns from DEAE-cellulose anion exchange chromatography. [3H]Glucosamine was incorporated equally into the two fractions.

Authors

James H. Shelhamer, Zvi Marom, Michael Kaliner