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Research Article Free access | 10.1172/JCI109939

Phorbol diester-induced macrophage differentiation of leukemic blasts from patients with human myelogenous leukemia.

H P Koeffler, M Bar-Eli, and M Territo

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Published November 1, 1980 - More info

Published in Volume 66, Issue 5 on November 1, 1980
J Clin Invest. 1980;66(5):1101–1108. https://doi.org/10.1172/JCI109939.
© 1980 The American Society for Clinical Investigation
Published November 1, 1980 - Version history
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Abstract

Phorbol esters, including 12-O-tetradecanoylphorbol 13-acetate (TPA), induce terminal macrophagelike differentiation of cells from human acute myelogenous leukemia lines. We report that myelogenous leukemia cells obtained from patients undergo macrophagelike differentiation after exposure to TPA. The myeloid leukemic cell cultured with TPA became adherent to charged surfaces with long filamentous pseudopodia; developed positive staining for alpha-napthyl acetate esterase, increased lysozyme secretion, reduced nitroblue tetrazolium, and acquired the ability to phagocytose candida. Cells from patients with lymphocytic leukemia did not become macrophagelike when cultured with TPA.

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