Go to JCI Insight
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Alerts
  • Advertising
  • Job board
  • Subscribe
  • Contact
  • Current issue
  • Past issues
  • By specialty
    • COVID-19
    • Cardiology
    • Gastroenterology
    • Immunology
    • Metabolism
    • Nephrology
    • Neuroscience
    • Oncology
    • Pulmonology
    • Vascular biology
    • All ...
  • Videos
    • Conversations with Giants in Medicine
    • Author's Takes
  • Reviews
    • View all reviews ...
    • Next-Generation Sequencing in Medicine (Upcoming)
    • New Therapeutic Targets in Cardiovascular Diseases (Mar 2022)
    • Immunometabolism (Jan 2022)
    • Circadian Rhythm (Oct 2021)
    • Gut-Brain Axis (Jul 2021)
    • Tumor Microenvironment (Mar 2021)
    • 100th Anniversary of Insulin's Discovery (Jan 2021)
    • View all review series ...
  • Viewpoint
  • Collections
    • In-Press Preview
    • Commentaries
    • Concise Communication
    • Editorials
    • Viewpoint
    • Top read articles
  • Clinical Medicine
  • JCI This Month
    • Current issue
    • Past issues

  • Current issue
  • Past issues
  • Specialties
  • Reviews
  • Review series
  • Conversations with Giants in Medicine
  • Author's Takes
  • In-Press Preview
  • Commentaries
  • Concise Communication
  • Editorials
  • Viewpoint
  • Top read articles
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Alerts
  • Advertising
  • Job board
  • Subscribe
  • Contact
Top
  • View PDF
  • Download citation information
  • Send a comment
  • Share this article
  • Terms of use
  • Standard abbreviations
  • Need help? Email the journal
  • Top
  • Abstract
  • Version history
  • Article usage
  • Citations to this article

Advertisement

Research Article Free access | 10.1172/JCI109584

Lipolysis Produces Changes in the Immunoreactivity and Cell Reactivity of Very Low Density Lipoproteins

G. Schonfeld, W. Patsch, B. Pfleger, J. L. Witztum, and S. W. Weidman

Lipid Research Center, Washington University School of Medicine, St. Louis, Missouri 63110

Department of Preventive Medicine, Washington University School of Medicine, St. Louis, Missouri 63110

Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110

Find articles by Schonfeld, G. in: JCI | PubMed | Google Scholar

Lipid Research Center, Washington University School of Medicine, St. Louis, Missouri 63110

Department of Preventive Medicine, Washington University School of Medicine, St. Louis, Missouri 63110

Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110

Find articles by Patsch, W. in: JCI | PubMed | Google Scholar

Lipid Research Center, Washington University School of Medicine, St. Louis, Missouri 63110

Department of Preventive Medicine, Washington University School of Medicine, St. Louis, Missouri 63110

Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110

Find articles by Pfleger, B. in: JCI | PubMed | Google Scholar

Lipid Research Center, Washington University School of Medicine, St. Louis, Missouri 63110

Department of Preventive Medicine, Washington University School of Medicine, St. Louis, Missouri 63110

Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110

Find articles by Witztum, J. in: JCI | PubMed | Google Scholar

Lipid Research Center, Washington University School of Medicine, St. Louis, Missouri 63110

Department of Preventive Medicine, Washington University School of Medicine, St. Louis, Missouri 63110

Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110

Find articles by Weidman, S. in: JCI | PubMed | Google Scholar

Published November 1, 1979 - More info

Published in Volume 64, Issue 5 on November 1, 1979
J Clin Invest. 1979;64(5):1288–1297. https://doi.org/10.1172/JCI109584.
© 1979 The American Society for Clinical Investigation
Published November 1, 1979 - Version history
View PDF
Abstract

Smaller very low density lipoprotein (VLDL) remnants interact more readily with tissues than do larger “intact” VLDL. This may be related to changes in the availability of VLDL apoproteins on the surface of the lipoproteins. To test this hypothesis VLDL were incubated at 37°C with bovine milk lipase (LPL), and the abilities of LPL-treated VLDL preparations to compete with 125I-low density lipoproteins (LDL) for interaction with cultured normal human fibroblasts were measured. At the same time, the immunologic activities of these preparations were also tested by double antibody radioimmunoassay. Triglyceride (TG) contents of VLDL fell by 30-90% during incubation with LPL and, on zonal ultracentrifugation, VLDL of faster Svedberg unit of flotation (Sf1.063) rates (>150) were gradually converted to smaller VLDL with lower Sf rates (21-60). LPL-treated VLDL competed two to five times more effectively with 125I-LDL for binding to cellular receptors than did control VLDL. Control VLDL incubated with heat-inactivated LPL at 37°C, or with active LPL at 4°C had unaltered cell reactivities and TG contents compared with VLDL incubated without any enzyme. The direct uptake and degradation of LPL-treated VLDL was also assessed by using VLDL 125I-labeled in apoprotein (Apo)B. LPL-treated VLDL-125I-ApoB were taken up and degraded by fibroblast at greater rates than were control VLDL-125I-ApoB. Thus, hydrolysis of VLDL lipids was accompanied by an increased ability of VLDL to interact with fibroblasts. The immunoreactivity of ApoB in the same VLDL preparations, expressed as the “apparent ApoB contents” of LPL-treated VLDL, increased by 10-50% (P < 0.02) in those assays that contained anti-LDL antisera, but the ApoB of control VLDL remained constant. However, assays that contained antisera directed against ApoB isolated from VLDL did not distinguish between LPL-treated and control VLDL. Thus, VLDL lipid hydrolysis was accompanied by changes in the immunoreactivity of VLDL-ApoB, which probably reflect changes in the disposition of ApoB on the surface of VLDL. The altered disposition of ApoB on VLDL “remnants” may be related to their enhanced interaction with cells.

Browse pages

Click on an image below to see the page. View PDF of the complete article

icon of scanned page 1288
page 1288
icon of scanned page 1289
page 1289
icon of scanned page 1290
page 1290
icon of scanned page 1291
page 1291
icon of scanned page 1292
page 1292
icon of scanned page 1293
page 1293
icon of scanned page 1294
page 1294
icon of scanned page 1295
page 1295
icon of scanned page 1296
page 1296
icon of scanned page 1297
page 1297
Version history
  • Version 1 (November 1, 1979): No description

Article tools

  • View PDF
  • Download citation information
  • Send a comment
  • Share this article
  • Terms of use
  • Standard abbreviations
  • Need help? Email the journal

Metrics

  • Article usage
  • Citations to this article

Go to

  • Top
  • Abstract
  • Version history
Advertisement
Advertisement

Copyright © 2022 American Society for Clinical Investigation
ISSN: 0021-9738 (print), 1558-8238 (online)

Sign up for email alerts