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Free access | 10.1172/JCI109227

Cell-Cell Interaction in Erythropoiesis: ROLE OF HUMAN MONOCYTES

John J. Rinehart, Esmail D. Zanjani, Benet Nomdedeu, Bobby J. Gormus, and Manuel E. Kaplan

Department of Medicine, University of Minnesota School of Medicine, Minneapolis, Minnesota 55417

Department of Physiology, University of Minnesota School of Medicine, Minneapolis, Minnesota 55417

Hematology Division, University of Minnesota School of Medicine, Minneapolis, Minnesota 55417

Veterans Administration Hospital, Minneapolis, Minnesota 55417

Find articles by Rinehart, J. in: PubMed | Google Scholar

Department of Medicine, University of Minnesota School of Medicine, Minneapolis, Minnesota 55417

Department of Physiology, University of Minnesota School of Medicine, Minneapolis, Minnesota 55417

Hematology Division, University of Minnesota School of Medicine, Minneapolis, Minnesota 55417

Veterans Administration Hospital, Minneapolis, Minnesota 55417

Find articles by Zanjani, E. in: PubMed | Google Scholar

Department of Medicine, University of Minnesota School of Medicine, Minneapolis, Minnesota 55417

Department of Physiology, University of Minnesota School of Medicine, Minneapolis, Minnesota 55417

Hematology Division, University of Minnesota School of Medicine, Minneapolis, Minnesota 55417

Veterans Administration Hospital, Minneapolis, Minnesota 55417

Find articles by Nomdedeu, B. in: PubMed | Google Scholar

Department of Medicine, University of Minnesota School of Medicine, Minneapolis, Minnesota 55417

Department of Physiology, University of Minnesota School of Medicine, Minneapolis, Minnesota 55417

Hematology Division, University of Minnesota School of Medicine, Minneapolis, Minnesota 55417

Veterans Administration Hospital, Minneapolis, Minnesota 55417

Find articles by Gormus, B. in: PubMed | Google Scholar

Department of Medicine, University of Minnesota School of Medicine, Minneapolis, Minnesota 55417

Department of Physiology, University of Minnesota School of Medicine, Minneapolis, Minnesota 55417

Hematology Division, University of Minnesota School of Medicine, Minneapolis, Minnesota 55417

Veterans Administration Hospital, Minneapolis, Minnesota 55417

Find articles by Kaplan, M. in: PubMed | Google Scholar

Published November 1, 1978 - More info

Published in Volume 62, Issue 5 on November 1, 1978
J Clin Invest. 1978;62(5):979–986. https://doi.org/10.1172/JCI109227.
© 1978 The American Society for Clinical Investigation
Published November 1, 1978 - Version history
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Abstract

Erythroid burst forming units (BFU-E) are proliferative cells present in peripheral blood and bone marrow which may be precursors of the erythroid colony forming cell found in the bone marrow. To examine the possible role of monocyte-macrophages in the modulation of erythropoiesis, the effect of monocytes on peripheral blood BFU-E proliferation in response to erythropoietin was investigated in the plasma clot culture system. Peripheral blood mononuclear cells from normal human donors were separated into four fractions. Fraction-I cells were obtained from the interface of Ficoll-Hypaque gradients (20-30% monocytes; 60-80% lymphocytes); fraction-II cells were fraction-I cells that were nonadherent to plastic (2-10% monocytes; 90-98% lymphocytes); fraction-III cells were obtained by incubation of fraction-II cells with carbonyl iron followed by Ficoll-Hypaque centrifugation (>99% lymphocytes); and fraction-IV cells represented the adherent population of fraction-II cells released from the plastic by lidocaine (>95% monocytes). When cells from these fractions were cultured in the presence of erythropoietin, the number of BFU-E-derived colonies was inversely proportional to the number of monocytes present (r = −0.96, P < 0.001). The suppressive effect of monocytes on BFU-E proliferation was confirmed by admixing autologous purified monocytes (fraction-IV cells) with fraction-III cells. Monocyte concentrations of ≥20% completely suppressed BFU-E activity. Reduction in the number of plated BFU-E by monocyte dilution could not account for these findings: a 15% reduction in the number of fraction-III cells plated resulted in only a 15% reduction in colony formation. These results indicate that monocyte-macrophages may play a significant role in the regulation of erythropoiesis and be involved in the pathogenesis of the hypoproliferative anemias associated with infection and certain neoplasia in which increased monocyte activity and monopoiesis also occur.

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