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Research Article Free access | 10.1172/JCI1089

The HPV-activating cellular transcription factor Brn-3a is overexpressed in CIN3 cervical lesions.

D Ndisdang, P J Morris, C Chapman, L Ho, A Singer, and D S Latchman

Windeyer Institute of Medical Sciences, University College London Medical School, The Windeyer Building, Cleveland Street, London W1P 6DB, United Kingdom.

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Windeyer Institute of Medical Sciences, University College London Medical School, The Windeyer Building, Cleveland Street, London W1P 6DB, United Kingdom.

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Windeyer Institute of Medical Sciences, University College London Medical School, The Windeyer Building, Cleveland Street, London W1P 6DB, United Kingdom.

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Windeyer Institute of Medical Sciences, University College London Medical School, The Windeyer Building, Cleveland Street, London W1P 6DB, United Kingdom.

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Windeyer Institute of Medical Sciences, University College London Medical School, The Windeyer Building, Cleveland Street, London W1P 6DB, United Kingdom.

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Windeyer Institute of Medical Sciences, University College London Medical School, The Windeyer Building, Cleveland Street, London W1P 6DB, United Kingdom.

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Published April 15, 1998 - More info

Published in Volume 101, Issue 8 on April 15, 1998
J Clin Invest. 1998;101(8):1687–1692. https://doi.org/10.1172/JCI1089.
© 1998 The American Society for Clinical Investigation
Published April 15, 1998 - Version history
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Abstract

The cervical cellular transcription factors Brn-3a and Brn-3b have antagonistic effects on transcription of the human papilloma virus types 16 and 18 E6 and E7 oncogenes, with Brn-3a activating expression and Brn-3b repressing it. We therefore measured expression of Brn-3a and Brn-3b mRNAs in biopsies from 16 women with no detectable cervical abnormality, and in 14 women with cervical intraepithelial neoplasia grade 3 (CIN3) lesions. Although the mean level of Brn-3b expression was similar in both groups, the mean level of Brn-3a expression was over 300-fold higher in the CIN3 samples when compared with normals. Elevated expression of Brn-3a was also detected in 16 histologically normal regions of the cervix adjacent to the CIN3 lesions, indicating that elevation of Brn-3a levels is not confined to the lesion in women with CIN3, and is thus not a consequence of the oncogenic process. The elevated levels of Brn-3a in the CIN3 patient samples, together with the activating effect of Brn-3a on HPV-16 and -18 oncogene expression, suggest that induction of this factor is involved in activating HPV-16 and -18 oncogene expression in the cervix, and hence in the production of cervical cancers induced by HPV.

Version history
  • Version 1 (April 15, 1998): No description
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