Usage Information

Isolation of a Novel Glycoprotein from the Urine of a Patient with Chronic Myelocytic Leukemia
Daniel Rudman, … , Alejandro E. Del Rio, Bettye Hollins
Daniel Rudman, … , Alejandro E. Del Rio, Bettye Hollins
Published March 1, 1974
Citation Information: J Clin Invest. 1974;53(3):868-874. https://doi.org/10.1172/JCI107627.
View: Text | PDF
Research Article

Isolation of a Novel Glycoprotein from the Urine of a Patient with Chronic Myelocytic Leukemia

Abstract

Patient B. J. with chronic myelocytic leukemia excreted 0.5-1.1 g protein per day in the urine. Gel filtration on Sephadex G-75 showed about one-third of this protein to be in molecular weight range 20,000-40,000 (fraction BJC). BJC, prepared from 9 liters of urine by gel filtration, was chromatographed on carboxymethylcellulose. Two proteins were eluted from the resin in pure form (as shown by zone and immunoelectrophoresis) in yields representing 8 and 3 mg/liter of urine: BJC1 and BJC2. Their amino acid compositions were identical. BJC1 contained 61% carbohydrate (33% hexose, 11% sialic acid, 13% glucosamine, 5% galactosamine). BJC2 contained one-fourth to one-half as much of each carbohydrate. Molecular weight of BJC1 was estimated at 29,000 by gel filtration. Neither glycoprotein reacted with rabbit antiserum to normal human serum.

Authors

Daniel Rudman, Rajender K. Chawla, Alejandro E. Del Rio, Bettye Hollins

×

Usage data is cumulative from July 2024 through July 2025.

Usage JCI PMC
Text version 112 7
PDF 109 5
Scanned page 276 0
Citation downloads 73 0
Totals 570 12
Total Views 582
(Click and drag on plot area to zoom in. Click legend items above to toggle)

Usage information is collected from two different sources: this site (JCI) and Pubmed Central (PMC). JCI information (compiled daily) shows human readership based on methods we employ to screen out robotic usage. PMC information (aggregated monthly) is also similarly screened of robotic usage.

Various methods are used to distinguish robotic usage. For example, Google automatically scans articles to add to its search index and identifies itself as robotic; other services might not clearly identify themselves as robotic, or they are new or unknown as robotic. Because this activity can be misinterpreted as human readership, data may be re-processed periodically to reflect an improved understanding of robotic activity. Because of these factors, readers should consider usage information illustrative but subject to change.

Advertisement