Quantitative Aspects of Pinocytosis and the Intracellular Fate of <i>N</i>-acetyl-α-D-glucosaminidase in Sanfilippo B Fibroblasts

K. von Figura; H. Kresse

doi:10.1172/JCI107563

Institute of Physiological Chemistry, University of Münster, D 44 Münster, West Germany

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Institute of Physiological Chemistry, University of Münster, D 44 Münster, West Germany

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Published in Volume 53, Issue 1 on January 1, 1974
J Clin Invest. 1974;53(1):85–90. https://doi.org/10.1172/JCI107563.
© 1974 The American Society for Clinical Investigation

Published January 1, 1974 - Version history

The cellular uptake of N-acetyl-α-D-glucosaminidase, the deficient enzyme in Sanfilippo B disease, and the intracellular fate and metabolic effect of this enzyme have been investigated in Sanfilippo B and normal fibroblasts. For both genotypes the uptake is highly efficient (up to 0.025 mU/h/mg cell protein), specific and constant over a period of at least 6 days. It is probable that the enzyme protein is taken up by adsorptive pinocytosis. The enzymatic activity as well as the biological activity towards ³⁵SO₄-labeled mucopolysaccharides persist in Sanfilippo B cells with a half-life of 34 h, indicating the intralysosomal localization of the pinocytosed enzyme.

The data obtained are discussed with regard to a possible enzyme replacement therapy. For Sanfilippo B disease the doses used in the past are considered to be insufficient to cause measurable effects.

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