Go to JCI Insight
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact
  • Clinical Research and Public Health
  • Current issue
  • Past issues
  • By specialty
    • COVID-19
    • Cardiology
    • Gastroenterology
    • Immunology
    • Metabolism
    • Nephrology
    • Neuroscience
    • Oncology
    • Pulmonology
    • Vascular biology
    • All ...
  • Videos
    • Conversations with Giants in Medicine
    • Video Abstracts
  • Reviews
    • View all reviews ...
    • Pancreatic Cancer (Jul 2025)
    • Complement Biology and Therapeutics (May 2025)
    • Evolving insights into MASLD and MASH pathogenesis and treatment (Apr 2025)
    • Microbiome in Health and Disease (Feb 2025)
    • Substance Use Disorders (Oct 2024)
    • Clonal Hematopoiesis (Oct 2024)
    • Sex Differences in Medicine (Sep 2024)
    • View all review series ...
  • Viewpoint
  • Collections
    • In-Press Preview
    • Clinical Research and Public Health
    • Research Letters
    • Letters to the Editor
    • Editorials
    • Commentaries
    • Editor's notes
    • Reviews
    • Viewpoints
    • 100th anniversary
    • Top read articles

  • Current issue
  • Past issues
  • Specialties
  • Reviews
  • Review series
  • Conversations with Giants in Medicine
  • Video Abstracts
  • In-Press Preview
  • Clinical Research and Public Health
  • Research Letters
  • Letters to the Editor
  • Editorials
  • Commentaries
  • Editor's notes
  • Reviews
  • Viewpoints
  • 100th anniversary
  • Top read articles
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact
Top
  • View PDF
  • Download citation information
  • Send a comment
  • Terms of use
  • Standard abbreviations
  • Need help? Email the journal
  • Top
  • Abstract
  • Version history
  • Article usage
  • Citations to this article

Advertisement

Research Article Free access | 10.1172/JCI107547

Blastogenic Suppression and Alloantibody Activity of Sera from Renal Allograft Recipients

Armead H. Johnson, Roger D. Rossen, Evan M. Hersh, Sandra S. Farrow, William T. Butler, and Wadi N. Suki

Department of Microbiology and Immunology, Baylor College of Medicine, Houston, Texas 77025

Department of Medicine, Baylor College of Medicine, Houston, Texas 77025

Department of Developmental Therapeutics, The University of Texas, M. D. Anderson Hospital and Tumor Institute, Houston, Texas 77025

The Methodist Hospital, Houston, Texas 77025

Veterans Administration Hospital, Houston, Texas 77025

Find articles by Johnson, A. in: PubMed | Google Scholar

Department of Microbiology and Immunology, Baylor College of Medicine, Houston, Texas 77025

Department of Medicine, Baylor College of Medicine, Houston, Texas 77025

Department of Developmental Therapeutics, The University of Texas, M. D. Anderson Hospital and Tumor Institute, Houston, Texas 77025

The Methodist Hospital, Houston, Texas 77025

Veterans Administration Hospital, Houston, Texas 77025

Find articles by Rossen, R. in: PubMed | Google Scholar

Department of Microbiology and Immunology, Baylor College of Medicine, Houston, Texas 77025

Department of Medicine, Baylor College of Medicine, Houston, Texas 77025

Department of Developmental Therapeutics, The University of Texas, M. D. Anderson Hospital and Tumor Institute, Houston, Texas 77025

The Methodist Hospital, Houston, Texas 77025

Veterans Administration Hospital, Houston, Texas 77025

Find articles by Hersh, E. in: PubMed | Google Scholar

Department of Microbiology and Immunology, Baylor College of Medicine, Houston, Texas 77025

Department of Medicine, Baylor College of Medicine, Houston, Texas 77025

Department of Developmental Therapeutics, The University of Texas, M. D. Anderson Hospital and Tumor Institute, Houston, Texas 77025

The Methodist Hospital, Houston, Texas 77025

Veterans Administration Hospital, Houston, Texas 77025

Find articles by Farrow, S. in: PubMed | Google Scholar

Department of Microbiology and Immunology, Baylor College of Medicine, Houston, Texas 77025

Department of Medicine, Baylor College of Medicine, Houston, Texas 77025

Department of Developmental Therapeutics, The University of Texas, M. D. Anderson Hospital and Tumor Institute, Houston, Texas 77025

The Methodist Hospital, Houston, Texas 77025

Veterans Administration Hospital, Houston, Texas 77025

Find articles by Butler, W. in: PubMed | Google Scholar

Department of Microbiology and Immunology, Baylor College of Medicine, Houston, Texas 77025

Department of Medicine, Baylor College of Medicine, Houston, Texas 77025

Department of Developmental Therapeutics, The University of Texas, M. D. Anderson Hospital and Tumor Institute, Houston, Texas 77025

The Methodist Hospital, Houston, Texas 77025

Veterans Administration Hospital, Houston, Texas 77025

Find articles by Suki, W. in: PubMed | Google Scholar

Published January 1, 1974 - More info

Published in Volume 53, Issue 1 on January 1, 1974
J Clin Invest. 1974;53(1):270–278. https://doi.org/10.1172/JCI107547.
© 1974 The American Society for Clinical Investigation
Published January 1, 1974 - Version history
View PDF
Abstract

To evaluate whether immunological enhancement plays a role in adaptation to renal allografts, we studied sera from transplant recipients to determine whether those which suppressed mixed leukocyte culture (MLC) responses in vitro contained alloantibodies reactive with donor cells. Sera from five of nine renal transplant recipients consistently and specifically suppressed autologous autologous MLC responses to donor cells without impairing the blastogenic responses to third-party leukocytes, soluble antigens, or nonspecific mitogenic agents. In three of the five cases the suppressive activity of the serum was striking; in two cases the effect was less marked but still readily demonstrable in studies designed to evaluate the dose of serum which provided optimal suppression of MLC responses. Serum from one of the recipients nonspecifically suppressed blastogenic activity both to donor cells and other stimuli. No alloantibody reactive with donor leukocytes was found in any of the sera which exhibited suppressive activity in MLC, whereas in one patient, serum which contained antibody reactive with donor cells did not suppress MLC response to that donor. These findings suggest that, if the serum factors which suppress MLC responses in vitro are enhancing antibodies, they are not detectable even with very sensitive techniques either because they are present in very low concentrations, belong to immunoglobulin classes other than IgA, IgG, or IgM, or are complexed with donor antigen in such a way that their ability to react with fresh donor cells in vitro is blocked.

Images.

Browse pages

Click on an image below to see the page. View PDF of the complete article

icon of scanned page 270
page 270
icon of scanned page 271
page 271
icon of scanned page 272
page 272
icon of scanned page 273
page 273
icon of scanned page 274
page 274
icon of scanned page 275
page 275
icon of scanned page 276
page 276
icon of scanned page 277
page 277
icon of scanned page 278
page 278
Version history
  • Version 1 (January 1, 1974): No description

Article tools

  • View PDF
  • Download citation information
  • Send a comment
  • Terms of use
  • Standard abbreviations
  • Need help? Email the journal

Metrics

  • Article usage
  • Citations to this article

Go to

  • Top
  • Abstract
  • Version history
Advertisement
Advertisement

Copyright © 2025 American Society for Clinical Investigation
ISSN: 0021-9738 (print), 1558-8238 (online)

Sign up for email alerts