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Research Article Free access | 10.1172/JCI107528

Regulation of Renal Cortex Ammoniagenesis I. STIMULATION OF RENAL CORTEX AMMONIAGENESIS IN VITRO BY PLASMA ISOLATED FROM ACUTELY ACIDOTIC RATS

George A. O. Alleyne and Anne Roobol

Department of Medicine, University of the West Indies, Mona, Kingston 7, Jamaica, West Indies

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Department of Medicine, University of the West Indies, Mona, Kingston 7, Jamaica, West Indies

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Published January 1, 1974 - More info

Published in Volume 53, Issue 1 on January 1, 1974
J Clin Invest. 1974;53(1):117–121. https://doi.org/10.1172/JCI107528.
© 1974 The American Society for Clinical Investigation
Published January 1, 1974 - Version history
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Abstract

We studied the acute renal metabolic response in rats made acidotic by a single oral dose of ammonium chloride. Cortical slices from acutely (2-h) acidotic rats utilized more glutamine and produced more ammonia and glucose from glutamine than slices from normal animals. When cortical slices from normal rats were pretreated in vitro with plasma isolated from acutely acidotic rats, they achieved similar increases in glutamine utilization, ammonia formation, and gluconeogenesis from glutamine. We did not observe such stimulation in normal cortical slices pretreated in a low pH-low bicarbonate medium. Our data show that a nondialysable factor is present in plasma from acutely acidotic rats that may be responsible for the early increase in the urinary ammonia observed in such animals.

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