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Research Article Free access | 10.1172/JCI107508

Biosynthesis of Proparathyroid Hormone and Parathyroid Hormone by Human Parathyroid Glands

Luke L. H. Chu, Ronal R. MacGregor, Paul I. Liu, James W. Hamilton, and David V. Cohn

Calcium Research Laboratory, Veterans Administration Hospital, Kansas City, Missouri 64128

University of Missouri-Kansas City, School of Dentistry, Kansas City, Missouri 64108

University of Kansas, School of Medicine, Kansas City, Kansas 66103

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Calcium Research Laboratory, Veterans Administration Hospital, Kansas City, Missouri 64128

University of Missouri-Kansas City, School of Dentistry, Kansas City, Missouri 64108

University of Kansas, School of Medicine, Kansas City, Kansas 66103

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Calcium Research Laboratory, Veterans Administration Hospital, Kansas City, Missouri 64128

University of Missouri-Kansas City, School of Dentistry, Kansas City, Missouri 64108

University of Kansas, School of Medicine, Kansas City, Kansas 66103

Find articles by Liu, P. in: PubMed | Google Scholar

Calcium Research Laboratory, Veterans Administration Hospital, Kansas City, Missouri 64128

University of Missouri-Kansas City, School of Dentistry, Kansas City, Missouri 64108

University of Kansas, School of Medicine, Kansas City, Kansas 66103

Find articles by Hamilton, J. in: PubMed | Google Scholar

Calcium Research Laboratory, Veterans Administration Hospital, Kansas City, Missouri 64128

University of Missouri-Kansas City, School of Dentistry, Kansas City, Missouri 64108

University of Kansas, School of Medicine, Kansas City, Kansas 66103

Find articles by Cohn, D. in: PubMed | Google Scholar

Published December 1, 1973 - More info

Published in Volume 52, Issue 12 on December 1, 1973
J Clin Invest. 1973;52(12):3089–3094. https://doi.org/10.1172/JCI107508.
© 1973 The American Society for Clinical Investigation
Published December 1, 1973 - Version history
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Abstract

Human parathyroid glands obtained at autopsy were incubated with [3H]leucine and [3H]lysine. After incubation, nonradioactive parathyroid tissue of either human or bovine origin was added. Radioactive parathyroid hormone and proparathyroid hormone were isolated from the gland and medium by organic solvent and salt fractionation, trichloroacetic acid precipitation, Sephadex G-100 gel filtration, and carboxymethyl cellulose column chromatography. The human hormonal peptides were identified in the ion-exchange column eluates by their relatively high levels of radioactivity, their elution positions, and their immunoreactivity to anti-PTH antiserum. The time-course of radioactive amino acid incorporation into these peptides and a brief incubation of the gland with radioactive amino acids, followed by various lengths of incubation with nonradioactive amino acids, indicated that a precursor-product relationship exists for the two peptides. An alternate method for isolation of the hormone and prohormone, which involves separation of peptides by urea-polyacrylamide gel electrophoresis, confirmed the identities of the human parathyroid hormone and proparathyroid hormone.

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Version history
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