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Research Article Free access | 10.1172/JCI107352
Department of Medicine, Baltimore City Hospitals, Baltimore 21224
Department of Medicine, The Johns Hopkins University, Baltimore, Maryland 21205
Department of Medicine, National Institutes of Health, Bethesda, Maryland 20014
Find articles by Guerrant, R. in: JCI | PubMed | Google Scholar
Department of Medicine, Baltimore City Hospitals, Baltimore 21224
Department of Medicine, The Johns Hopkins University, Baltimore, Maryland 21205
Department of Medicine, National Institutes of Health, Bethesda, Maryland 20014
Find articles by Ganguly, U. in: JCI | PubMed | Google Scholar
Department of Medicine, Baltimore City Hospitals, Baltimore 21224
Department of Medicine, The Johns Hopkins University, Baltimore, Maryland 21205
Department of Medicine, National Institutes of Health, Bethesda, Maryland 20014
Find articles by Casper, A. in: JCI | PubMed | Google Scholar
Department of Medicine, Baltimore City Hospitals, Baltimore 21224
Department of Medicine, The Johns Hopkins University, Baltimore, Maryland 21205
Department of Medicine, National Institutes of Health, Bethesda, Maryland 20014
Find articles by Moore, E. in: JCI | PubMed | Google Scholar
Department of Medicine, Baltimore City Hospitals, Baltimore 21224
Department of Medicine, The Johns Hopkins University, Baltimore, Maryland 21205
Department of Medicine, National Institutes of Health, Bethesda, Maryland 20014
Find articles by Pierce, N. in: JCI | PubMed | Google Scholar
Department of Medicine, Baltimore City Hospitals, Baltimore 21224
Department of Medicine, The Johns Hopkins University, Baltimore, Maryland 21205
Department of Medicine, National Institutes of Health, Bethesda, Maryland 20014
Find articles by Carpenter, C. in: JCI | PubMed | Google Scholar
Published July 1, 1973 - More info
An Escherichia coli strain isolated from a patient with severe cholera-like diarrhea elaborates a partly heat-labile enterotoxin shown to cause prompt adenyl cyclase stimulation and isotonic fluid secretion by canine jejunum. Both responses disappear upon removal of the enterotoxin. The duration of action of a submaximal dose of this E. coli enterotoxin was brief, despite sustained exposure to the jejunum, suggesting inactivation of the enterotoxin by its interaction with the mucosa.
Inoculation of whole bacterial cultures of this E. coli strain into canine duodenum was followed by bacterial survival and induction of net secretion after 4-7 h. The onset of fluid production was associated with increasing gut mucosal adenyl cyclase activity. Washed bacterial cells could also produce fluid secretion. In vivo multiplication of this enterotoxin-producing E. coli was demonstrated 6-12 h after intraduodenal inoculation of approximately 106 organisms. This was associated with fluid secretion. Intestinal fluid production occurred without microscopic pathology in the mucosa.
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