Go to JCI Insight
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact
  • Clinical Research and Public Health
  • Current issue
  • Past issues
  • By specialty
    • COVID-19
    • Cardiology
    • Gastroenterology
    • Immunology
    • Metabolism
    • Nephrology
    • Neuroscience
    • Oncology
    • Pulmonology
    • Vascular biology
    • All ...
  • Videos
    • Conversations with Giants in Medicine
    • Video Abstracts
  • Reviews
    • View all reviews ...
    • Complement Biology and Therapeutics (May 2025)
    • Evolving insights into MASLD and MASH pathogenesis and treatment (Apr 2025)
    • Microbiome in Health and Disease (Feb 2025)
    • Substance Use Disorders (Oct 2024)
    • Clonal Hematopoiesis (Oct 2024)
    • Sex Differences in Medicine (Sep 2024)
    • Vascular Malformations (Apr 2024)
    • View all review series ...
  • Viewpoint
  • Collections
    • In-Press Preview
    • Clinical Research and Public Health
    • Research Letters
    • Letters to the Editor
    • Editorials
    • Commentaries
    • Editor's notes
    • Reviews
    • Viewpoints
    • 100th anniversary
    • Top read articles

  • Current issue
  • Past issues
  • Specialties
  • Reviews
  • Review series
  • Conversations with Giants in Medicine
  • Video Abstracts
  • In-Press Preview
  • Clinical Research and Public Health
  • Research Letters
  • Letters to the Editor
  • Editorials
  • Commentaries
  • Editor's notes
  • Reviews
  • Viewpoints
  • 100th anniversary
  • Top read articles
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact
Top
  • View PDF
  • Download citation information
  • Send a comment
  • Terms of use
  • Standard abbreviations
  • Need help? Email the journal
  • Top
  • Abstract
  • Version history
  • Article usage
  • Citations to this article

Advertisement

Research Article Free access | 10.1172/JCI107318

In Vitro Antibody-Enzyme Conjugates with Specific Bactericidal Activity

Daniel M. Knowles II, Timothy J. Sullivan III, Charles W. Parker, and Ralph C. Williams Jr.

Department of Medicine, Bernalillo County Medical Center, University of New Mexico School of Medicine, Albuquerque, New Mexico 87131

Department of Medicine, Washington University School of Medicine, Barnes and Wohl Hospitals, St. Louis, Missouri 63110

Find articles by Knowles, D. in: PubMed | Google Scholar

Department of Medicine, Bernalillo County Medical Center, University of New Mexico School of Medicine, Albuquerque, New Mexico 87131

Department of Medicine, Washington University School of Medicine, Barnes and Wohl Hospitals, St. Louis, Missouri 63110

Find articles by Sullivan, T. in: PubMed | Google Scholar

Department of Medicine, Bernalillo County Medical Center, University of New Mexico School of Medicine, Albuquerque, New Mexico 87131

Department of Medicine, Washington University School of Medicine, Barnes and Wohl Hospitals, St. Louis, Missouri 63110

Find articles by Parker, C. in: PubMed | Google Scholar

Department of Medicine, Bernalillo County Medical Center, University of New Mexico School of Medicine, Albuquerque, New Mexico 87131

Department of Medicine, Washington University School of Medicine, Barnes and Wohl Hospitals, St. Louis, Missouri 63110

Find articles by Williams, R. in: PubMed | Google Scholar

Published June 1, 1973 - More info

Published in Volume 52, Issue 6 on June 1, 1973
J Clin Invest. 1973;52(6):1443–1452. https://doi.org/10.1172/JCI107318.
© 1973 The American Society for Clinical Investigation
Published June 1, 1973 - Version history
View PDF
Abstract

IgG with antibacterial antibody opsonic activity was isolated from rabbit antisera produced by intravenous hyperimmunization with several test strains of pneumococci, Group A β-hemolytic streptococci, Staphylococcus aureus, Proteus mirabilis, Pseudomonas aeruginosa, and Escherichia coli. Antibody-enzyme conjugates were prepared, using diethylmalonimidate to couple glucose oxidase to IgG antibacterial antibody preparations. Opsonic human IgG obtained from serum of patients with subacute bacterial endocarditis was also conjugated to glucose oxidase. Antibody-enzyme conjugates retained combining specificity for test bacteria as demonstrated by indirect immunofluorescence. In vitro test for bactericidal activity of antibody-enzyme conjugates utilized potassium iodide, lactoperoxidase, and glucose as cofactors. Under these conditions glucose oxidase conjugated to antibody generates hydrogen peroxide, and lactoperoxidase enzyme catalyzes the reduction of hydrogen peroxide with simultaneous oxidation of I- and halogenation and killing of test bacteria. Potent in vitro bactericidal activity of this system was repeatedly demonstrated for antibody-enzyme conjugates against pneumococci, streptococci, S. aureus, P. mirabilis, and E. coli. However, no bactericidal effect was demonstrable with antibody-enzyme conjugates and two test strains of P. aeruginosa. Bactericidal activity of antibody-enzyme conjugates appeared to parallel original opsonic potency of unconjugated IgG preparations. Antibody-enzyme conjugates at concentrations as low as 0.01 mg/ml were capable of intense bactericidal activity producing substantial drops in surviving bacterial counts within 30-60 min after initiation of assay. These in vitro bactericidal systems indicate that the concept of antibacterial antibody-enzyme conjugates may possibly be adaptable as a mechanism for treatment of patients with leukocyte dysfunction or fulminant bacteremia.

Browse pages

Click on an image below to see the page. View PDF of the complete article

icon of scanned page 1443
page 1443
icon of scanned page 1444
page 1444
icon of scanned page 1445
page 1445
icon of scanned page 1446
page 1446
icon of scanned page 1447
page 1447
icon of scanned page 1448
page 1448
icon of scanned page 1449
page 1449
icon of scanned page 1450
page 1450
icon of scanned page 1451
page 1451
icon of scanned page 1452
page 1452
Version history
  • Version 1 (June 1, 1973): No description

Article tools

  • View PDF
  • Download citation information
  • Send a comment
  • Terms of use
  • Standard abbreviations
  • Need help? Email the journal

Metrics

  • Article usage
  • Citations to this article

Go to

  • Top
  • Abstract
  • Version history
Advertisement
Advertisement

Copyright © 2025 American Society for Clinical Investigation
ISSN: 0021-9738 (print), 1558-8238 (online)

Sign up for email alerts