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Research Article Free access | 10.1172/JCI107316

Tissue Factor Activity in Lymphocyte Cultures from Normal Individuals and Patients with Hemophilia A

Frederick R. Rickles, John A. Hardin, Frances A. Pitlick, Leon W. Hoyer, and Marcel E. Conrad

Department of Hematology, Walter Reed Army Institute of Research, Walter Reed Army Medical Center, Washington, D. C. 20012

National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20014

Department of Medicine, Yale University School of Medicine, New Haven, Connecticut 06510

Department of Medicine, University of Connecticut School of Medicine, Farmington, Connecticut

Veterans Administration Hospital, Newington, Connecticut 06111

Find articles by Rickles, F. in: JCI | PubMed | Google Scholar

Department of Hematology, Walter Reed Army Institute of Research, Walter Reed Army Medical Center, Washington, D. C. 20012

National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20014

Department of Medicine, Yale University School of Medicine, New Haven, Connecticut 06510

Department of Medicine, University of Connecticut School of Medicine, Farmington, Connecticut

Veterans Administration Hospital, Newington, Connecticut 06111

Find articles by Hardin, J. in: JCI | PubMed | Google Scholar

Department of Hematology, Walter Reed Army Institute of Research, Walter Reed Army Medical Center, Washington, D. C. 20012

National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20014

Department of Medicine, Yale University School of Medicine, New Haven, Connecticut 06510

Department of Medicine, University of Connecticut School of Medicine, Farmington, Connecticut

Veterans Administration Hospital, Newington, Connecticut 06111

Find articles by Pitlick, F. in: JCI | PubMed | Google Scholar

Department of Hematology, Walter Reed Army Institute of Research, Walter Reed Army Medical Center, Washington, D. C. 20012

National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20014

Department of Medicine, Yale University School of Medicine, New Haven, Connecticut 06510

Department of Medicine, University of Connecticut School of Medicine, Farmington, Connecticut

Veterans Administration Hospital, Newington, Connecticut 06111

Find articles by Hoyer, L. in: JCI | PubMed | Google Scholar

Department of Hematology, Walter Reed Army Institute of Research, Walter Reed Army Medical Center, Washington, D. C. 20012

National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20014

Department of Medicine, Yale University School of Medicine, New Haven, Connecticut 06510

Department of Medicine, University of Connecticut School of Medicine, Farmington, Connecticut

Veterans Administration Hospital, Newington, Connecticut 06111

Find articles by Conrad, M. in: JCI | PubMed | Google Scholar

Published June 1, 1973 - More info

Published in Volume 52, Issue 6 on June 1, 1973
J Clin Invest. 1973;52(6):1427–1434. https://doi.org/10.1172/JCI107316.
© 1973 The American Society for Clinical Investigation
Published June 1, 1973 - Version history
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Abstract

The procoagulant material of lymphocytes has been characterized as tissue factor. Lymphocytes stimulated with phytohemagglutinin or the purified protein derivative of the tubercle bacillus developed procoagulant activity with incubation in tissue culture. While this material corrected the prolonged clotting time of factor VIII (AHF) deficient plasma, we have shown, utilizing a sensitive radioimmunoassay, that no AHF antigen was present in the cell cultures. Further, we have demonstrated this material to be tissue factor by coagulation techniques and immunological cross-reactivity. The published data regarding factor VIII synthesis is reviewed in light of these observations and comments are made regarding the role of the lymphocyte procoagulant.

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