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Research Article Free access | 10.1172/JCI107284

Purification and Biophysical Characterization of Hepatitis B Antigen

Chung Yong Kim and Jeremiah G. Tilles

Thorndike Memorial Laboratory, Boston City Hospital, Boston 02118

Harvard Medical Services, Boston City Hospital, Boston 02118

Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115

Find articles by Kim, C. in: PubMed | Google Scholar

Thorndike Memorial Laboratory, Boston City Hospital, Boston 02118

Harvard Medical Services, Boston City Hospital, Boston 02118

Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115

Find articles by Tilles, J. in: PubMed | Google Scholar

Published May 1, 1973 - More info

Published in Volume 52, Issue 5 on May 1, 1973
J Clin Invest. 1973;52(5):1176–1186. https://doi.org/10.1172/JCI107284.
© 1973 The American Society for Clinical Investigation
Published May 1, 1973 - Version history
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Abstract

Hepatitis B antigen (HB Ag) has been purified from serum by pepsin digestion and equilibrium sedimentation. The sedimentation coefficient, density, particle size, and electrophoretic mobility have been determined before and after purification and found to be unaltered. In addition, the diffusion constant and molecular weight of purified HB Ag have been documented.

Because the biophysical characteristics found are not consistant with those of any known virus, it is believed that native HB Ag is made up almost entirely of incomplete virus.

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