Effects of carbon tetrachloride on albumin synthesis

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Abstract

The effects of CCl4 on albumin synthesis were studied employing the isolated perfused liver. Carbonate-14C was used to measure newly synthesized albumin. 2.5 ml of CCl4 was administered by stomach tube 2 hr before perfusion. Albumin synthesis decreased from 36 to 5 mg following the ingestion of CCl4. Preperfusing the livers for 1 hr before measuring albumin synthesis resulted in an increase to 12 mg, and the addition of tryptophan to a final concentration of 10 mM resulted in a further increase to 19 mg. Cortisone did not protect against the toxic effects of CCl4 when administered to the donor rabbits. Fasting resulted in an increased sensitivity to CCl4 and an antioxidant was not effective in protecting against the toxic manifestations of CCl4.

Authors

Marcus A. Rothschild, Murray Oratz, Sidney S. Schreiber