Bile Acid Synthesis in Man: Metabolism of 7α-Hydroxycholesterol-<sup>14</sup>C and 26-Hydroxycholesterol-<sup>3</sup>H

Karl E. Anderson; Engeline Kok; Norman B. Javitt

doi:10.1172/JCI106780

Bile Acid Synthesis in Man: Metabolism of 7α-Hydroxycholesterol-¹⁴C and 26-Hydroxycholesterol-³H

Karl E. Anderson, Engeline Kok, and Norman B. Javitt

Published January 1, 1972 - More info

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Abstract

The pathways of bile acid synthesis in man were evaluated by studying the metabolism of 7α-hydroxycholesterol-4-¹⁴C and 26-hydroxycholesterol-16, 22-³H administered parenterally to individuals requiring external biliary drainage. Techniques for the identification of metabolites were thin-layer chromatography, column chromatography, gas-liquid chromatography with stream splitting, and crystallization to constant specific activity. It was found that both compounds were rapidly metabolized to bile acids and excreted in bile. Of the total radioactivity recovered in bile as bile acids, 87% of the 26-hydroxycholesterol-³H and 90% of the 7α-hydroxycholesterol-¹⁴C was found to be metabolized to both chenodeoxycholate and cholate. Compared to 7α-hydroxycholesterol, a greater proportion of 26-hydroxycholesterol was found to be metabolized to chenodeoxycholate.

These findings indicate that both 7α-hydroxycholesterol and 26-hydroxycholesterol can be intermediates in the metabolism of cholesterol to bile acids in man. The observation that conversion to cholate takes place less readily after C-26 hydroxylation is consistent with previous findings in other species.