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Research Article Free access | 10.1172/JCI106762

Quantitative assessment of the extent of myocardial infarction in the conscious dog by means of analysis of serial changes in serum creatine phosphokinase activity

William E. Shell, John K. Kjekshus, and Burton E. Sobel

Department of Medicine, School of Medicine, University of California, San Diego, California 92103

La Jolla, California 92037

Find articles by Shell, W. in: PubMed | Google Scholar

Department of Medicine, School of Medicine, University of California, San Diego, California 92103

La Jolla, California 92037

Find articles by Kjekshus, J. in: PubMed | Google Scholar

Department of Medicine, School of Medicine, University of California, San Diego, California 92103

La Jolla, California 92037

Find articles by Sobel, B. in: PubMed | Google Scholar

Published December 1, 1971 - More info

Published in Volume 50, Issue 12 on December 1, 1971
J Clin Invest. 1971;50(12):2614–2625. https://doi.org/10.1172/JCI106762.
© 1971 The American Society for Clinical Investigation
Published December 1, 1971 - Version history
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Abstract

This study was designed to develop a method for quantitative assessment of infarct size in the conscious animal based on serial changes of serum creatine phosphokinase (CPK) activity. From 11 experiments in which myocardial CPK was injected intravenously in conscious dogs, the average CPK distribution space and average CPK fractional disappearance rate from serum were found to be 11.4% of body weight and 0.48% min respectively. In other experiments, myocardial infarction was produced in 22 conscious dogs by constriction of a left coronary artery snare and serum CPK activity was determined at frequent intervals for 24 hr. Since myocardial CPK depletion reflects infarct size, infarct size was determined directly by analysis of myocardial CPK content in the same animals 24 hr after coronary artery occlusion. CPK released from the infarct was determined from observed changes in serum CPK activity analyzed according to a model taking into account the fraction of CPK released from an infarct and the rates of appearance and disappearance of CPK activity from serum. Infarct size was calculated on the basis of observed changes in serum CPK and compared to infarct size determined directly by analysis of myocardial CPK depletion. Agreement was close and results from all experiments fit the equation: [infarct size (g) determined from serum CPK] = 1.13 × [infarct size (g) determined from myocardial CPK] - 1.3, r = 0.96, n = 22. The method described is useful for accurate assessment of infarct size in the conscious animal and for detection of modification of infarct size produced by pharmacologic interventions.

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