Influence of antidiuretic hormone on peritoneal membrane area and permeability

Lee W. Henderson; James E. Kintzel

doi:10.1172/JCI106742

Influence of antidiuretic hormone on peritoneal membrane area and permeability

Lee W. Henderson and James E. Kintzel

Published November 1, 1971 - More info

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Abstract

The present study was carried out to determine if antidiuretic hormone (ADH) altered the solute handling characteristics of the peritoneal membrane. Lightly anesthetized dogs primed with urea-¹⁴C (60 mol wt) and inulin (5200 mol wt) were volume expanded with hypotonic saline solution to suppress endogenous ADH as assessed by urine/plasma osmolality. With ADH suppressed, two to three control peritoneal dialysis exchanges were carried out. A constant infusion of ADH in a physiologic dose of 150 mU/hr in saline was begun and the urine/plasma osmolality followed until it was significantly greater than one. Two to three experimental dialysis exchanges were then carried out. Dialysance across the peritoneal membrane was calculated for inulin (D_I) and urea (D_U). In 16 such studies D_U fell in all but three (the mean value for the fall was 2.8 ±2.6 ml/min; P < 0.001). D_I varied randomly and showed no significant change. In all 16 studies D_I/D_U rose (D_I/D_U = 0.054 ± 0.054; P < 0.005). Seven dogs were studied with an identical protocol but saline was infused without ADH. D_U and the dialysance ratio varied randomly. D_U fell in one and did not change or rose in four and D_I/D_U rose in two and fell in three. The data are interpreted to show a fall in area but an increase in mean pore radius of the “peritoneal membrane” in response to physiologic amounts of intravenous ADH. The fall in area is consistent with a decreasing splanchnic blood flow.