Catalase-dependent peroxidative metabolism in the alveolar macrophage during phagocytosis

J. Bernard L. Gee; Charles L. Vassallo; Paul Bell; James Kaskin; R. E. Basford; James B. Field

doi:10.1172/JCI106340

^{Department of Medicine University of Pittsburgh School of Medicine and Veterans Administration Hospital, Pittsburgh, Pennsylvania 15213}

^{Department of Biochemistry, University of Pittsburgh School of Medicine and Veterans Administration Hospital, Pittsburgh, Pennsylvania 15213}

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^{Department of Medicine University of Pittsburgh School of Medicine and Veterans Administration Hospital, Pittsburgh, Pennsylvania 15213}

^{Department of Biochemistry, University of Pittsburgh School of Medicine and Veterans Administration Hospital, Pittsburgh, Pennsylvania 15213}

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^{Department of Medicine University of Pittsburgh School of Medicine and Veterans Administration Hospital, Pittsburgh, Pennsylvania 15213}

^{Department of Biochemistry, University of Pittsburgh School of Medicine and Veterans Administration Hospital, Pittsburgh, Pennsylvania 15213}

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^{Department of Medicine University of Pittsburgh School of Medicine and Veterans Administration Hospital, Pittsburgh, Pennsylvania 15213}

^{Department of Biochemistry, University of Pittsburgh School of Medicine and Veterans Administration Hospital, Pittsburgh, Pennsylvania 15213}

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^{Department of Medicine University of Pittsburgh School of Medicine and Veterans Administration Hospital, Pittsburgh, Pennsylvania 15213}

^{Department of Biochemistry, University of Pittsburgh School of Medicine and Veterans Administration Hospital, Pittsburgh, Pennsylvania 15213}

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^{Department of Medicine University of Pittsburgh School of Medicine and Veterans Administration Hospital, Pittsburgh, Pennsylvania 15213}

^{Department of Biochemistry, University of Pittsburgh School of Medicine and Veterans Administration Hospital, Pittsburgh, Pennsylvania 15213}

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Published in Volume 49, Issue 6 on June 1, 1970
J Clin Invest. 1970;49(6):1280–1287. https://doi.org/10.1172/JCI106340.
© 1970 The American Society for Clinical Investigation

Published June 1, 1970 - Version history

Evidence for the presence of peroxidative metabolism in rabbit alveolar macrophages (AM) has been obtained from the following observations: (a) catalase is present in high concentrations; (b) peroxidase activity could not be detected employing guaiacol as substrate; (c) the irreversible inhibition of AM catalase by aminotriazole served as a detection system for H₂O₂ and demonstrated increased intracellular H₂O₂ after phagocytosis; (d) formate oxidation, a marker of catalase-dependent peroxidations, occurs in resting AM and is increased by phagocytosis; (c) measurements of H₂O₂ accumulation in a dialysate of AM demonstrated twofold increase during phagocytosis; and (f) aminotriazole diminishes O₂ utilization and ¹⁴CO₂ production from labelled glucose and pyruvate. It is concluded that, while catalase-dependent H₂O₂ metabolism is not essential for particle entry, this pathway represents one of the metabolic pathways stimulated by particle entry in the AM.

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Catalase-dependent peroxidative metabolism in the alveolar macrophage during phagocytosis

J. Bernard L. Gee, Charles L. Vassallo, Paul Bell, James Kaskin, R. E. Basford, and James B. Field

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Catalase-dependent peroxidative metabolism in the alveolar macrophage during phagocytosis

J. Bernard L. Gee, Charles L. Vassallo, Paul Bell, James Kaskin, R. E. Basford, and James B. Field

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