Go to JCI Insight
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact
  • Clinical Research and Public Health
  • Current issue
  • Past issues
  • By specialty
    • COVID-19
    • Cardiology
    • Gastroenterology
    • Immunology
    • Metabolism
    • Nephrology
    • Neuroscience
    • Oncology
    • Pulmonology
    • Vascular biology
    • All ...
  • Videos
    • Conversations with Giants in Medicine
    • Video Abstracts
  • Reviews
    • View all reviews ...
    • Complement Biology and Therapeutics (May 2025)
    • Evolving insights into MASLD and MASH pathogenesis and treatment (Apr 2025)
    • Microbiome in Health and Disease (Feb 2025)
    • Substance Use Disorders (Oct 2024)
    • Clonal Hematopoiesis (Oct 2024)
    • Sex Differences in Medicine (Sep 2024)
    • Vascular Malformations (Apr 2024)
    • View all review series ...
  • Viewpoint
  • Collections
    • In-Press Preview
    • Clinical Research and Public Health
    • Research Letters
    • Letters to the Editor
    • Editorials
    • Commentaries
    • Editor's notes
    • Reviews
    • Viewpoints
    • 100th anniversary
    • Top read articles

  • Current issue
  • Past issues
  • Specialties
  • Reviews
  • Review series
  • Conversations with Giants in Medicine
  • Video Abstracts
  • In-Press Preview
  • Clinical Research and Public Health
  • Research Letters
  • Letters to the Editor
  • Editorials
  • Commentaries
  • Editor's notes
  • Reviews
  • Viewpoints
  • 100th anniversary
  • Top read articles
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact
Top
  • View PDF
  • Download citation information
  • Send a comment
  • Terms of use
  • Standard abbreviations
  • Need help? Email the journal
  • Top
  • Abstract
  • Version history
  • Article usage
  • Citations to this article

Advertisement

Research Article Free access | 10.1172/JCI106249

The effects of deoxygenation of adult and fetal hemoglobin on the synthesis of red cell 2,3-diphosphoglycerate and its in vivo consequences

Frank A. Oski, Arlan J. Gottlieb, William W. Miller, and Maria Delivoria-Papadopoulos

Department of Pediatrics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19146

Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19146

Department of Physiology, Graduate Division of the University of Pennsylvania School of Medicine, Hospital of the University of Pennsylvania and The Children's Hospital of Philadelphia, Philadelphia, Pennsylvania 19146

Find articles by Oski, F. in: PubMed | Google Scholar

Department of Pediatrics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19146

Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19146

Department of Physiology, Graduate Division of the University of Pennsylvania School of Medicine, Hospital of the University of Pennsylvania and The Children's Hospital of Philadelphia, Philadelphia, Pennsylvania 19146

Find articles by Gottlieb, A. in: PubMed | Google Scholar

Department of Pediatrics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19146

Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19146

Department of Physiology, Graduate Division of the University of Pennsylvania School of Medicine, Hospital of the University of Pennsylvania and The Children's Hospital of Philadelphia, Philadelphia, Pennsylvania 19146

Find articles by Miller, W. in: PubMed | Google Scholar

Department of Pediatrics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19146

Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19146

Department of Physiology, Graduate Division of the University of Pennsylvania School of Medicine, Hospital of the University of Pennsylvania and The Children's Hospital of Philadelphia, Philadelphia, Pennsylvania 19146

Find articles by Delivoria-Papadopoulos, M. in: PubMed | Google Scholar

Published February 1, 1970 - More info

Published in Volume 49, Issue 2 on February 1, 1970
J Clin Invest. 1970;49(2):400–407. https://doi.org/10.1172/JCI106249.
© 1970 The American Society for Clinical Investigation
Published February 1, 1970 - Version history
View PDF
Abstract

Patients over 1 month of age with arterial oxygen pressures of less than 60 mm Hg were found to have elevated red cell 2,3-diphosphoglycerate (2,3-DPG) levels and blood with a decreased affinity for oxygen. The increase in 2,3-DPG was proportional to the degree of hypoxemia. In patients under 1 month of age this relationship was not observed. Red cells from adults, but not newborns, showed rapid increases in 2,3-DPG when incubated under nitrogen. Adult, but not fetal, deoxyhemoglobin was shown to facilitate in vitro synthesis of 2,3-DPG by binding this organic phosphate and relieving the product inhibition of 2,3-DPG mutase.

Throughout a wide range change in oxygen affinity as measured by the P50 is linear with respect to the 2,3-DPG concentration; a change of 430 mμmoles of 2,3-DPG/ml of red blood corpuscles (RBC) resulting in a change of the P50 of 1 mm Hg. It appears that the 2,3-DPG of the adult's red cells responds rapidly to metabolic and environmental influences and in turn effects metabolism and the cellular environment. Many of these effects are not shared by the red cells of the newborn.

Browse pages

Click on an image below to see the page. View PDF of the complete article

icon of scanned page 400
page 400
icon of scanned page 401
page 401
icon of scanned page 402
page 402
icon of scanned page 403
page 403
icon of scanned page 404
page 404
icon of scanned page 405
page 405
icon of scanned page 406
page 406
icon of scanned page 407
page 407
Version history
  • Version 1 (February 1, 1970): No description

Article tools

  • View PDF
  • Download citation information
  • Send a comment
  • Terms of use
  • Standard abbreviations
  • Need help? Email the journal

Metrics

  • Article usage
  • Citations to this article

Go to

  • Top
  • Abstract
  • Version history
Advertisement
Advertisement

Copyright © 2025 American Society for Clinical Investigation
ISSN: 0021-9738 (print), 1558-8238 (online)

Sign up for email alerts