Nonsuppressible insulin-like activity in human serum: III. Differentiation of two distinct molecules with nonsuppressible ILA

• Text
• PDF

Abstract

Total nonsuppressible insulin-like activity (ILA) of human plasma (measured by the adipose tissue assay) results from the additive effects of at least two distinct components. They differ in molecular size, solubility in acid-ethanol, and in thermostability. More than 90% of nonsuppressible ILA of human plasma is insoluble in acid-ethanol. Its molecular size of 100,000-150,000 remains unchanged by treatment with acid-ethanol, 5 M acetic acid-0.15 M NaCl, urea, and EDTA. It is inactivated by heat.

Authors

A. Jakob, Ch. Hauri, E. R. Froesch