Usage Information

Incorporation of orthophosphate-32P into erythrocyte phospholipids in normal subjects and in patients with hereditary spherocytosis
Claude F. Reed
Claude F. Reed
Published December 1, 1968
Citation Information: J Clin Invest. 1968;47(12):2630-2638. https://doi.org/10.1172/JCI105946.
View: Text | PDF
Research Article

Incorporation of orthophosphate-32P into erythrocyte phospholipids in normal subjects and in patients with hereditary spherocytosis

Abstract

The in vitro incorporation of inorganic 32P into erythrocyte phospholipids has been studied in normal subjects and in splenectomized patients with hereditary spherocytosis (HS). Phosphatidic acid (PA) was the only lipid measurably labeled in both kinds of cells. The actual turnover rate of PA phosphate was determined by simultaneously isolating inorganic phosphate (Pi) and adenosine triphosphate (ATP) and determining their specific activities. This turnover is very small: 1.3 μmoles P/liter of erythrocytes per hr in normal cells and 4.0 μmoles P in HS erythrocytes when either ATP or cellular Pi is considered the immediate precursor. This value represents less than 0.1% of the total membrane lipid phosphate. Incorporation of added 32Pi into the other phosphatides, including phosphatidyl serine, was essentially zero in both kinds of cells.

Authors

Claude F. Reed

×

Usage data is cumulative from July 2024 through July 2025.

Usage JCI PMC
Text version 140 1
PDF 85 5
Scanned page 398 2
Citation downloads 107 0
Totals 730 8
Total Views 738
(Click and drag on plot area to zoom in. Click legend items above to toggle)

Usage information is collected from two different sources: this site (JCI) and Pubmed Central (PMC). JCI information (compiled daily) shows human readership based on methods we employ to screen out robotic usage. PMC information (aggregated monthly) is also similarly screened of robotic usage.

Various methods are used to distinguish robotic usage. For example, Google automatically scans articles to add to its search index and identifies itself as robotic; other services might not clearly identify themselves as robotic, or they are new or unknown as robotic. Because this activity can be misinterpreted as human readership, data may be re-processed periodically to reflect an improved understanding of robotic activity. Because of these factors, readers should consider usage information illustrative but subject to change.

Advertisement