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Research Article Free access | 10.1172/JCI105844

Variations in purine metabolism of cultured skin fibroblasts from patients with gout

J. Frank Henderson, Frederick M. Rosenbloom, William N. Kelley, and J. Edwin Seegmiller

1National Institute of Arthritis and Metabolic Diseases, National Institutes of Health, Bethesda, Maryland 20014

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1National Institute of Arthritis and Metabolic Diseases, National Institutes of Health, Bethesda, Maryland 20014

Find articles by Rosenbloom, F. in: PubMed | Google Scholar

1National Institute of Arthritis and Metabolic Diseases, National Institutes of Health, Bethesda, Maryland 20014

Find articles by Kelley, W. in: PubMed | Google Scholar

1National Institute of Arthritis and Metabolic Diseases, National Institutes of Health, Bethesda, Maryland 20014

Find articles by Seegmiller, J. in: PubMed | Google Scholar

Published July 1, 1968 - More info

Published in Volume 47, Issue 7 on July 1, 1968
J Clin Invest. 1968;47(7):1511–1516. https://doi.org/10.1172/JCI105844.
© 1968 The American Society for Clinical Investigation
Published July 1, 1968 - Version history
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Abstract

Purine metabolism was studied in fibroblasts cultured from three patients with gout in an attempt to determine the biochemical bases of their disease. The rate of purine biosynthesis de novo was normal in one line of cells, but the rate of catabolism of adenine nucleotides to hypoxanthine and inosine was greatly increased. The rate of purine biosynthesis de novo was increased in two lines of cells, and this was associated with increased concentrations of 5-phosphoribosyl 1-pyrophosphate. Purine synthesis was also less sensitive than normal to feedback inhibition. The catabolism of inosinate synthesized de novo was increased.

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