Studies of Paroxysmal Nocturnal Hemoglobinuria Erythrocytes: Increased Lysis and Lipid Peroxide Formation by Hydrogen Peroxide

Charles E. Mengel; Herbert E. Kann; Wilhelm D. Meriwether

doi:10.1172/JCI105662

Department of Medicine, The Ohio State University, College of Medicine, Columbus, Ohio

‡

Markle Scholar in Academic Medicine, Director, Division of Hematology and Oncology, The Ohio State University, Columbus, Ohio.

Research Fellow, Division of Hematology and Oncology, The Ohio State University, Columbus, Ohio.

Research Trainee, Division of Hematology and Oncology, The Ohio State University University Hospitals, Columbus, Ohio, and Medical Student, Duke University School of Medicine, Durham, N. C.

Address requests for reprints to Dr. Charles E. Mengel, Division of Hematology and Oncology, The Department of Medicine, The Ohio State University, College of Medicine, Columbus, Ohio 43210.

Received for publication 10 November 1966 and in revised form 10 July 1967.

Presented in part before the Central Society for Clinical Research, November 1965, Chicago, Ill.

Supported by U. S. Public Health Service Research Grants CA-08699 and CA-08702, and by U. S. N. Research Contract Nonr-495 (30), and U. S. Public Health Service Training Grant CA-5192-01.

Find articles by Mengel, C. in: PubMed | Google Scholar

Department of Medicine, The Ohio State University, College of Medicine, Columbus, Ohio

‡

Markle Scholar in Academic Medicine, Director, Division of Hematology and Oncology, The Ohio State University, Columbus, Ohio.

Research Fellow, Division of Hematology and Oncology, The Ohio State University, Columbus, Ohio.

Research Trainee, Division of Hematology and Oncology, The Ohio State University University Hospitals, Columbus, Ohio, and Medical Student, Duke University School of Medicine, Durham, N. C.

Address requests for reprints to Dr. Charles E. Mengel, Division of Hematology and Oncology, The Department of Medicine, The Ohio State University, College of Medicine, Columbus, Ohio 43210.

Received for publication 10 November 1966 and in revised form 10 July 1967.

Presented in part before the Central Society for Clinical Research, November 1965, Chicago, Ill.

Supported by U. S. Public Health Service Research Grants CA-08699 and CA-08702, and by U. S. N. Research Contract Nonr-495 (30), and U. S. Public Health Service Training Grant CA-5192-01.

Find articles by Kann, H. in: PubMed | Google Scholar

Department of Medicine, The Ohio State University, College of Medicine, Columbus, Ohio

‡

Markle Scholar in Academic Medicine, Director, Division of Hematology and Oncology, The Ohio State University, Columbus, Ohio.

Research Fellow, Division of Hematology and Oncology, The Ohio State University, Columbus, Ohio.

Research Trainee, Division of Hematology and Oncology, The Ohio State University University Hospitals, Columbus, Ohio, and Medical Student, Duke University School of Medicine, Durham, N. C.

Address requests for reprints to Dr. Charles E. Mengel, Division of Hematology and Oncology, The Department of Medicine, The Ohio State University, College of Medicine, Columbus, Ohio 43210.

Received for publication 10 November 1966 and in revised form 10 July 1967.

Presented in part before the Central Society for Clinical Research, November 1965, Chicago, Ill.

Supported by U. S. Public Health Service Research Grants CA-08699 and CA-08702, and by U. S. N. Research Contract Nonr-495 (30), and U. S. Public Health Service Training Grant CA-5192-01.

Find articles by Meriwether, W. in: PubMed | Google Scholar

When paroxysmal nocturnal hemoglobinuria (PNH) erythrocytes were exposed to H₂O₂ they lysed excessively and formed greater than normal quantities of lipid peroxides when compared to red cells of normal subjects and patients with most types of hematologic disease. It was also shown that lytic sensitivity to acidified serum was related to the enhanced lytic sensitivity to H₂O₂. If the lipid of PNH cells was first extracted then exposed to ultraviolet radiation more lipid peroxides were formed than in extracts of normal red blood cells. The possible explanations for these findings and their relationship to the PNH hemolytic mechanism are discussed.

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