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Research Article Free access | 10.1172/JCI105634

Studies of the Metabolic Activity of Leukocytes from Patients with a Genetic Abnormality of Phagocytic Function

Beulah Holmes, Arthur R. Page, and Robert A. Good

Pediatrics Research Laboratories of the Variety Club Heart Hospital, Minneapolis, Minnesota

Department of Pediatrics, University of Minnesota, Minneapolis, Minnesota

Department of Microbiology, University of Minnesota, Minneapolis, Minnesota

‡

Research Assistant, U. S. Public Health Service Training Grant in Medical Microbiology AI 90-06.

Address requests for reprints to Dr. Beulah Holmes, Department of Microbiology, University of Minnesota Medical School, Minneapolis, Minn. 55455.

§

Associate Professor, Career Development Awardee of the U. S. Public Health Service.

ǁ

American Legion Memorial Heart Research Professor of Pediatrics and Microbiology.

*

Submitted for publication 18 January 1967; accepted 5 June 1967.

Supported by the U. S. Public Health Service grants (AM-05830) (HE-06314), AI-00798 and The National Foundation.

Presented in part at the Sixth Annual Meeting, Society for Cell Biology, 1966.

Find articles by Holmes, B. in: PubMed | Google Scholar

Pediatrics Research Laboratories of the Variety Club Heart Hospital, Minneapolis, Minnesota

Department of Pediatrics, University of Minnesota, Minneapolis, Minnesota

Department of Microbiology, University of Minnesota, Minneapolis, Minnesota

‡

Research Assistant, U. S. Public Health Service Training Grant in Medical Microbiology AI 90-06.

Address requests for reprints to Dr. Beulah Holmes, Department of Microbiology, University of Minnesota Medical School, Minneapolis, Minn. 55455.

§

Associate Professor, Career Development Awardee of the U. S. Public Health Service.

ǁ

American Legion Memorial Heart Research Professor of Pediatrics and Microbiology.

*

Submitted for publication 18 January 1967; accepted 5 June 1967.

Supported by the U. S. Public Health Service grants (AM-05830) (HE-06314), AI-00798 and The National Foundation.

Presented in part at the Sixth Annual Meeting, Society for Cell Biology, 1966.

Find articles by Page, A. in: PubMed | Google Scholar

Pediatrics Research Laboratories of the Variety Club Heart Hospital, Minneapolis, Minnesota

Department of Pediatrics, University of Minnesota, Minneapolis, Minnesota

Department of Microbiology, University of Minnesota, Minneapolis, Minnesota

‡

Research Assistant, U. S. Public Health Service Training Grant in Medical Microbiology AI 90-06.

Address requests for reprints to Dr. Beulah Holmes, Department of Microbiology, University of Minnesota Medical School, Minneapolis, Minn. 55455.

§

Associate Professor, Career Development Awardee of the U. S. Public Health Service.

ǁ

American Legion Memorial Heart Research Professor of Pediatrics and Microbiology.

*

Submitted for publication 18 January 1967; accepted 5 June 1967.

Supported by the U. S. Public Health Service grants (AM-05830) (HE-06314), AI-00798 and The National Foundation.

Presented in part at the Sixth Annual Meeting, Society for Cell Biology, 1966.

Find articles by Good, R. in: PubMed | Google Scholar

Published September 1, 1967 - More info

Published in Volume 46, Issue 9 on September 1, 1967
J Clin Invest. 1967;46(9):1422–1432. https://doi.org/10.1172/JCI105634.
© 1967 The American Society for Clinical Investigation
Published September 1, 1967 - Version history
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Abstract

Polymorphonuclear leukocytes from patients with chronic granulomatous disease respond to the phagocytosis of latex particles with normal increments in glucose consumption, lactate production, Krebs' cycle activity, and lipid turnover.

The leukocytes of these patients fail to show normal increments in respiration, direct oxidation of glucose, and hydrogen peroxide formation during particle uptake.

It appears that the stimulation of respiration with the formation of hydrogen peroxide and stimulation of the direct oxidative pathway of glucose metabolism are closely linked to degranulation and intracellular killing of bacteria by polymorphonuclear leukocytes.

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