Submit a comment

Interactions between Antibiotics and Human Neutrophils in the Killing of Staphylococci: STUDIES WITH NORMAL AND CYTOCHALASIN B-TREATED CELLS
Richard K. Root, … , Julia A. Metcalf, Harry L. Malech
Richard K. Root, … , Julia A. Metcalf, Harry L. Malech
Published January 1, 1981
Citation Information: J Clin Invest. 1981;67(1):247-259. https://doi.org/10.1172/JCI110020.
View: Text | PDF

Interactions between Antibiotics and Human Neutrophils in the Killing of Staphylococci: STUDIES WITH NORMAL AND CYTOCHALASIN B-TREATED CELLS

Abstract

Normal and antibiotic-pretreated staphylococci were incubated with human neutrophils to determine the interactions between cells and antimicrobials in the killing of the organisms. Staphylococcus aureus 502A pretreated during log-phase growth with subinhibitory (¼ minimum inhibiting concentration) (MIC) concentrations of penicillin G were more susceptible to killing by normal neutrophils than untreated bacteria (intracellular survival 0.17±0.04 vs. 1.5±0.38%, mean±SEM, respectively, at 35 min in 14 experiments; P < 0.01 by t test). Furthermore, this enhanced susceptibility to killing was observed even when phagosome formation was inhibited by cytochalasin B (65.6±4.6% pencillintreated vs. 30.5±4.5% untreated killed at 30 min in 14 experiments, P < 0.001). Pretreatment of S. aureus with vancomycin similarly enhanced susceptibility to killing by cytochalasin B-treated polymorphonuclear leukocytes (PMN), whereas pretreatment with gentamicin did not.

Authors

Richard K. Root, Raul Isturiz, Abdolghader Molavi, Julia A. Metcalf, Harry L. Malech

×

Guidelines

The Editorial Board will only consider comments that are deemed relevant and of interest to readers. The Journal will not post data that have not been subjected to peer review; or a comment that is essentially a reiteration of another comment.

  • Comments appear on the Journal’s website and are linked from the original article’s web page.
  • Authors are notified by email if their comments are posted.
  • The Journal reserves the right to edit comments for length and clarity.
  • No appeals will be considered.
  • Comments are not indexed in PubMed.

Specific requirements

  • Maximum length, 400 words
  • Entered as plain text or HTML
  • Author’s name and email address, to be posted with the comment
  • Declaration of all potential conflicts of interest (even if these are not ultimately posted); see the Journal’s conflict-of-interest policy
  • Comments may not include figures
This field is required
This field is required
This field is required
This field is required
This field is required
This field is required