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Metabolism of the stimulated rat spleen: I. Ferrochelatase activity as an index of tissue erythropoiesis
Abraham Mazur
Abraham Mazur
Published October 1, 1968
Citation Information: J Clin Invest. 1968;47(10):2230-2238. https://doi.org/10.1172/JCI105908.
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Metabolism of the stimulated rat spleen: I. Ferrochelatase activity as an index of tissue erythropoiesis

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Abstract

Assay of the enzyme ferrochelatase in marrow, liver, spleen, and red cells has been employed to assess the extent of erythropoietic stimulation in animals bearing the Walker 256 carcinosarcoma and in rats treated by administration of phenylhydrazine, cobalt chloride, human urinary erythropoietin, or chronic blood loss. In all instances, the spleen sustains the most marked increase of ferrochelatase activity, per gram of tissue. Spleen erythropoietic activity stimulation was confirmed by quantitative measurements in respiring slices of 59Fe and 14C incorporation into hemoglobin and ferritin. Increased spleen ferrochelatase activity in cobalt chloride-treated rats is prevented by actinomycin D, indicating that stimulated synthesis of the enzyme is associated with the metabolism of RNA.

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Abraham Mazur

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