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Infusion of mature megakaryocytes into mice yields functional platelets
Rudy Fuentes, … , M. Anna Kowalska, Mortimer Poncz
Rudy Fuentes, … , M. Anna Kowalska, Mortimer Poncz
Published October 25, 2010
Citation Information: J Clin Invest. 2010;120(11):3917-3922. https://doi.org/10.1172/JCI43326.
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Brief Report

Infusion of mature megakaryocytes into mice yields functional platelets

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Abstract

Thrombopoiesis, the process by which circulating platelets arise from megakaryocytes, remains incompletely understood. Prior studies suggest that megakaryocytes shed platelets in the pulmonary vasculature. To better understand thrombopoiesis and to develop a potential platelet transfusion strategy that is not dependent upon donors, of which there remains a shortage, we examined whether megakaryocytes infused into mice shed platelets. Infused megakaryocytes led to clinically relevant increases in platelet numbers. The released platelets were normal in size, displayed appropriate surface markers, and had a near-normal circulating half-life. The functionality of the donor-derived platelets was also demonstrated in vivo. The infused megakaryocytes mostly localized to the pulmonary vasculature, where they appeared to shed platelets. These data suggest that it may be unnecessary to generate platelets from ex vivo grown megakaryocytes to achieve clinically relevant increases in platelet numbers.

Authors

Rudy Fuentes, Yuhuan Wang, Jessica Hirsch, Cheng Wang, Lubica Rauova, G. Scott Worthen, M. Anna Kowalska, Mortimer Poncz

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Figure 3

Organ distribution studies of infused cells.

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Organ distribution studies of infused cells.
(A) Staining of lung from h...
(A) Staining of lung from hαIIb+ mice infused with saline or small or large cells grown in BrdU (arrows point to stained nuclei). (B) Kinetics of BrdU-labeled large cells in the lungs. (C) Same as in A but for spleen. Scale bars (A–C): 200 μm. (D) Large FL cells 30 minutes after infusion in lungs: BrdU-labeled nuclei (left) and mαIIb (right). Scale bars: 50 μm. Data are representative of 3 separate studies.
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