Immunofluorescent staining revealed the cellular location of the actin-binding proteins ankyrin, spectrin, and Na⁺/K⁺-ATPase in cadaveric transplanted kidneys with prompt graft function (PGF) and delayed graft function (DGF) (35). The stained sections were examined at ×60–600 magnification using a scanning laser confocal microscope. Scoring was done as follows: 0, continuous staining confined to the basolateral membrane; 0.5, 1.0, and 1.5, interrupted linear staining of basolateral membrane with less than 50%, approximately 50%, and greater than 50% of the staining, respectively, appearing in the cytoplasm. In kidneys with delayed graft function, approximately 50% of the ankyrin, spectrin, and Na⁺/K⁺-ATPase was translocated from the basolateral membrane to the cytoplasm, whereas those kidneys with prompt graft function had only minimal translocation of these proteins from the basolateral membrane. *P < 0.01 vs. PGF, **P < 0.05 vs. PGF.