Messenger RNA reprogramming by spliceosome-mediated RNA trans-splicing
Mariano A. Garcia-Blanco
Mariano A. Garcia-Blanco
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Messenger RNA reprogramming by spliceosome-mediated RNA trans-splicing

Abstract

In the human genome, the majority of protein-encoding genes are interrupted by introns, which are removed from primary transcripts by a macromolecular enzyme known as the spliceosome. Spliceosomes can constitutively remove all the introns in a primary transcript to yield a fully spliced mRNA or alternatively splice primary transcripts leading to the production of many different mRNAs from one gene. This review examines how spliceosomes can recombine two primary transcripts in trans to reprogram messenger RNAs.

Authors

Mariano A. Garcia-Blanco

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A schematic of two mRNA variants derived from alternative splicing of a ...
A schematic of two mRNA variants derived from alternative splicing of a primary transcript. In situation i, exon 2 is recognized whereas exon 3 is not and is read as being part of a large intron. In contrast, the reverse is true for situation ii. In many instances, alternative splicing is tightly regulated in cell type–specific fashion (27). An, refers to the polyA tail at the 3′ end of mRNAs.