(A) Relative Hsd11b1, Cyp11b1, and Cyp11b2 expression (corrected for Gapdh) in tumor cells was determined by reverse-transcription qPCR. (B) 2.5 × 10⁴ tumor cells were cultured with 100 nM DOC or DHC for 24 hours, and steroids were assayed by ELISAs (Arbor Assays). (C) Relative gene expression in human cancers. TCGA gene expression data were analyzed using Gene Expression Profiling Interactive Analysis (http://gepia.cancer-pku.cn/), and median values are shown.