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Free access | 10.1172/JCI109989

Polycythemia Vera: FURTHER IN VITRO STUDIES OF HEMATOPOIETIC REGULATION

John W. Adamson, Jack W. Singer, Pat Catalano, Scott Murphy, Nancy Lin, Laura Steinmann, Connie Ernst, and Philip J. Fialkow

Section of Hematology, Medical Service, Veterans Administration Medical Center and University of Washington, Seattle, Washington 98195

Section of Oncology, Medical Service, Veterans Administration Medical Center and University of Washington, Seattle, Washington 98195

Section of Medical Genetics, Medical Service, Veterans Administration Medical Center and University of Washington, Seattle, Washington 98195

The Cardeza Foundation, Thomas Jefferson University, Philadelphia, Pennsylvania 19107

Find articles by Adamson, J. in: PubMed | Google Scholar

Section of Hematology, Medical Service, Veterans Administration Medical Center and University of Washington, Seattle, Washington 98195

Section of Oncology, Medical Service, Veterans Administration Medical Center and University of Washington, Seattle, Washington 98195

Section of Medical Genetics, Medical Service, Veterans Administration Medical Center and University of Washington, Seattle, Washington 98195

The Cardeza Foundation, Thomas Jefferson University, Philadelphia, Pennsylvania 19107

Find articles by Singer, J. in: PubMed | Google Scholar

Section of Hematology, Medical Service, Veterans Administration Medical Center and University of Washington, Seattle, Washington 98195

Section of Oncology, Medical Service, Veterans Administration Medical Center and University of Washington, Seattle, Washington 98195

Section of Medical Genetics, Medical Service, Veterans Administration Medical Center and University of Washington, Seattle, Washington 98195

The Cardeza Foundation, Thomas Jefferson University, Philadelphia, Pennsylvania 19107

Find articles by Catalano, P. in: PubMed | Google Scholar

Section of Hematology, Medical Service, Veterans Administration Medical Center and University of Washington, Seattle, Washington 98195

Section of Oncology, Medical Service, Veterans Administration Medical Center and University of Washington, Seattle, Washington 98195

Section of Medical Genetics, Medical Service, Veterans Administration Medical Center and University of Washington, Seattle, Washington 98195

The Cardeza Foundation, Thomas Jefferson University, Philadelphia, Pennsylvania 19107

Find articles by Murphy, S. in: PubMed | Google Scholar

Section of Hematology, Medical Service, Veterans Administration Medical Center and University of Washington, Seattle, Washington 98195

Section of Oncology, Medical Service, Veterans Administration Medical Center and University of Washington, Seattle, Washington 98195

Section of Medical Genetics, Medical Service, Veterans Administration Medical Center and University of Washington, Seattle, Washington 98195

The Cardeza Foundation, Thomas Jefferson University, Philadelphia, Pennsylvania 19107

Find articles by Lin, N. in: PubMed | Google Scholar

Section of Hematology, Medical Service, Veterans Administration Medical Center and University of Washington, Seattle, Washington 98195

Section of Oncology, Medical Service, Veterans Administration Medical Center and University of Washington, Seattle, Washington 98195

Section of Medical Genetics, Medical Service, Veterans Administration Medical Center and University of Washington, Seattle, Washington 98195

The Cardeza Foundation, Thomas Jefferson University, Philadelphia, Pennsylvania 19107

Find articles by Steinmann, L. in: PubMed | Google Scholar

Section of Hematology, Medical Service, Veterans Administration Medical Center and University of Washington, Seattle, Washington 98195

Section of Oncology, Medical Service, Veterans Administration Medical Center and University of Washington, Seattle, Washington 98195

Section of Medical Genetics, Medical Service, Veterans Administration Medical Center and University of Washington, Seattle, Washington 98195

The Cardeza Foundation, Thomas Jefferson University, Philadelphia, Pennsylvania 19107

Find articles by Ernst, C. in: PubMed | Google Scholar

Section of Hematology, Medical Service, Veterans Administration Medical Center and University of Washington, Seattle, Washington 98195

Section of Oncology, Medical Service, Veterans Administration Medical Center and University of Washington, Seattle, Washington 98195

Section of Medical Genetics, Medical Service, Veterans Administration Medical Center and University of Washington, Seattle, Washington 98195

The Cardeza Foundation, Thomas Jefferson University, Philadelphia, Pennsylvania 19107

Find articles by Fialkow, P. in: PubMed | Google Scholar

Published December 1, 1980 - More info

Published in Volume 66, Issue 6 on December 1, 1980
J Clin Invest. 1980;66(6):1363–1368. https://doi.org/10.1172/JCI109989.
© 1980 The American Society for Clinical Investigation
Published December 1, 1980 - Version history
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Abstract

Further in vitro studies of hematopoietic regulation were carried out in two patients with polycythemia vera who were also heterozygotes (GdB/GdA) for glucose-6-phosphate-dehydrogenase (G-6-PD). While only G-6-PD type A was detectable in circulating erythrocytes, granulocytes and platelets, cultures of peripheral blood and marrow from one patient revealed a substantial number of G-6-PD type B erythroid burst-forming units (BFU-E) and granulocyte/macrophage colony-forming units. Detailed analysis demonstrated: (a) where detectable, normal BFU-E and granulocyte/macrophage colony-forming units were found with similar frequencies; (b) the same frequencies for normal progenitors characterized cultures of peripheral blood and marrow; (c) inhibition of normal erythroid differentiation between BFU-E and the more mature erythroid colony-forming unit; (d) a decline in the prevalence of normal colony-forming units with time, suggesting that disease progression is associated with further suppression of normal hematopoiesis by products of the abnormal clone.

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